The Dutt laboratory focuses on understanding the genetic changes that occur in human cancer cells and how these changes can help develop the next generation of effective targeted therapies and diagnostics to improve treatment of cancer patients, using innovative computational and functional approaches.
The goal of Dutt laboratory is to understand the somatic genetics of human cancer and help develop the next generation of effective targeted therapies to improve treatment of cancer patients. The group specifically focuses on the genomic features of genetic alterations underlying oncogenesis and cancer progression in the lung, breast, cervical, gallbladder, head and neck, and others cancers. The efforts involve using advanced sequencing methodologies followed by functional validation to study the implications of genetic alterations in identifying novel cancer dependencies, therapeutic strategies, and biomarkers.
Cancer Genomics: Using computational approaches we analyze genome wide alterations underlying the cancer genome such as point mutations, complex structural alterations, and aberrant transcript fusion and expression. We develop robust in-house automated computational pipelines and analytical tools to analyze the high throughput datasets generated by whole exome, transcriptome and genome sequencing such as Waterhose, ClinOme, Cancer Pathogen Detector, HPVDetector, DepRanker,RVD, COVID qPCR Analyzer and others. We recently developed TMC-SNPdb - the first Indian germline SNPdb.
Functional Genomics: The genome-discovery efforts made in the laboratory are paired with functional validation using biochemical, molecular, cell based experimental approaches, and transgenic mouse models. We perturb the candidate target genes using CRISPR-Cas system and perform genome wide pooled shRNA screen using tumor derived cell lines to complement and validate our findings from genomic approaches. Specifically, the lab focusses on EGFR, FGFR and NOTCH family pathways.
Pathogen Discovery: We have developed a computational pipeline, HPVDetector to detect the presence of HPV DNA sequences in human clinical cancer samples. Next we used a similar aproach to detect the presence of non-typhoidal Salmonella in gallbladder cancer. We are currently expanding our endeavor to explore the presence of several other pathogen sequences in the human cancer genome using Cancer Pathogen Detector (CPD), an improved version of the computation pipeline. Simultaneouly, we are in process to deploy the CPD application as a “Container” onto the host cloud system (like CGC) using Docker system.
A randomized study carried out at Tata Memorial Centre showed a disease-free and overall survival benefit for pr… more
Pre-operative progesterone treatment confers survival benefit to node-positive breast cancer patients. Our recen… more
Breast cancer is most common cancer in India. Of the total incidence of breast cancer in India, about 50 percent… more
Tongue cancer is the most predominant form of oral cancer in developed countries with varying incidence in devel… more
Nodal metastases status among early stage tongue cancer patients (pT1-pT2) plays a decisive role for choice of t… more
Lung cancer remains the leading cause of cancer related deaths across the globe. According to the Globocan, arou… more
Treatment with tyrosine kinase inhibitors (TKIs) is the standard of care for lung cancer patients positive for m… more
Lung cancer resistance is the major obstacle to targeted therapies involving tyrosine kinase inhibitors (TKI). I… more
Treatment of cancers like NSCLC has revolutionized in the last decade owing to the identification of driver muta… more
The area of my research is to characterize the genomic landscape of somatic alterations in cervical cancer from… more
The uncommonness of gallbladder cancer in the developed world has contributed to the generally poor understandin… more
We are exploring the genomic landscape of papillary thyroid cancer and medullary thyroid cancer in Indian patien… more
Cancer is a complex disease characterized by somatic alterations (genetic and epigenetic), pathogenic infections… more
Pathogenic infections have been associated with cancer worldwide. About 18-20% of the malignancies have been att… more
We have developed a fully automated, robust computational pipeline called Waterhose, for analysis of high throug… more
Scientific Assistant E
Madhugiri V, Moiyadi AV, Shetty P, Gupta T, Epari S, Jalali R, Subeikshanan V, Dutt A, Sasidharan G, Kumar V, Ganesh C V Ramesh A, Prabhu A (2021)
Dharavath B, Yadav N, Desai S, Sunder R, Mishra R, Ketkar M, Bhanshe P, Gupta A, Redhu AK, Patkar N, Dutt S, Gupta S, Dutt A.
Kaur E, Nair J, Ghorai A, Mishra SV, Achareker A, Ketkar M, Sarkar D, Salunkhe S, Rajendra J, Gardi N, Desai S, Iyer P, Thorat R, Dutt A, Moiyadi A, Dutt S.
Noronha V, Patil VM, Joshi A, Menon N, Chougule A, Mahajan A, Janu A, Purandare N, Kumar R, More S, Goud S, Kadam N, Daware N, Bhattacharjee A, Shah S, Yadav A, Trivedi V, Behel V, Dutt A, Banavali SD, Prabhash K