Digital Imaging

Facility Team:

• Mrs. Vaishali Kailaje, Scientific Assistant,’F’ , Facility In charge
• Mrs. Tanuja Durve, Scientific Assistant, ‘E’
• Ms. Rajashree Sawant, Scientific Assistant, ‘B’

Facility Board Members:

• Dr. Sorab Dalal, Scientific Officer, ‘G’, Deputy Director, CRI
• Dr. Rohan Khadilkar, Scientific Officer, ‘E’

Facility Reporting Officer:

• Mr. Uday Dandekar. Engineer, ‘H’

Student’s representatives:

• Ms. Shivali Mishra
• Mr. Sourav Chakraborty

ZEISS LSM 980 AS2 Confocal

The ZEISS LSM 980 with Airyscan 2 is a next-generation laser scanning confocal microscope designed for high-sensitivity, and super-resolution fluorescence imaging. Featuring the innovative Airyscan 2 detector, the system captures significantly more light and spatial information than conventional confocal microscopes, delivering enhanced resolution, superior signal-to-noise ratio, and improved imaging speed with minimal phototoxicity. Integrated with automated stage control, Definite Focus technology, spectral detection, and live-cell imaging capabilities, the LSM 980 is an ideal platform for advanced biological research.

     

CONFOCAL SYSTEMS Nikon AX Confocal

The Nikon AX Confocal Microscope is a state-of-the-art laser scanning confocal imaging system engineered for high-speed, high-resolution, and large field-of-view imaging. Built on the Nikon Ti2-Eclipse fully motorized inverted microscope, with 25 mm ultra-large field-of-view galvo scanner, up to 8192 × 8192-pixel image acquisition, imaging speeds up to 10 fps, 360° scan field rotation, Perfect Focus System (PFS) for long-term live-cell imaging, environmental incubator for 35 mm dishes and multiwell plates.

     

LSM780 Confocal

 

• High-resolution fluorescence imaging of cells and tissues
• Optical sectioning and three-dimensional (3D) reconstruction of cells, tissues, organoids spheroids etc.
• Live-cell and short and long-term time-lapse imaging to study migration, division, autophagy and dynamics of organelles and proteins.
• Multi-color fluorescence imaging
• Protein localization and intracellular trafficking studies
• Colocalization and interaction analysis of biomolecules (FRET)
• Fluorescence Recovery After Photobleaching (FRAP) to study mobility and diffusion of proteins.
• Cell morphology and cytoskeletal organization studies
• Organelle imaging (mitochondria, nucleus, lysosomes, Golgi, ER, etc.)
• Cancer biology and tumor microenvironment research
• Drug screening and pharmacological studies
• Large-area tissue imaging using tile scanning
• Quantitative fluorescence intensity measurements
• Biomaterial and scaffold imaging
• Reflection imaging of non-fluorescent specimens

WIDEFIELD SYSTEMS - Axio Imager Z.1 Upright Microscope, Carl Zeiss

The AxioImager Z.1 is an upright fluorescence microscope used for imaging of cells, tissues, and diverse biological specimens. Partially motorized components, equipped with ZEISS optics - 6 position emission filter wheel and objective turret.

     

BX63, Upright Microscope, Olympus

The Olympus BX63 is a partially motorized upright microscope used for imaging of biological specimens - cells, tissues, organism etc. with 8 position emission filter wheel, LED as illumination sources for fluorescence light.

     

IX73 Inverted Microscope, Olympus

The Olympus IX73 is a compact inverted fluorescence microscope designed for routine cell culture observation and basic fluorescence imaging. Equipped with phase contrast, fluorescence illumination, and a color digital camera

   

Axio Observer Inverted Microscope, Carl Zeiss

The Carl Zeiss Axio Observer is a fully motorized inverted fluorescence microscope used for advanced live-cell and fluorescence imaging. Featuring an environmental chamber, Colibri LED illumination and Definite focus for z-drift compensation.

     

Nikon Eclipse Ti Inverted Microscope

Nikon Eclipse Ti Inverted Microscope is a Live-cell and fluorescence imaging system. Equipped with an environmental chamber that maintains physiological conditions (37°C and 5% CO₂), the system enables long-term imaging of living cells. It has the Perfect Focus for Z drift compensation.

 

Incucyte

This is a widefield inverted live cell system engineered with long term high throughput imaging in mind. Housed inside a tissue culture incubator, the imaging module maintains the most stable imaging conditions possible (37ºC temperature, humidity and 5% CO2). The system comes with a selection of inserts allowing you to image your cells in a range of vessels (ranging from 35mm dishes to 384 well plates). It automatically captures and analyses images of living cells in real time for days, weeks, or months. It has a optical design wherein the cells remain stationary while optics move. Multiple imaging modes – HD phase plus red and green fluorescence. It can accommodate multiple users at a time with different experiment set ups and configurations

           

Dedicated analysis workstations equipped with Imaris, Arivis Pro

Imaris for advanced 3D visualization, segmentation, cell tracking, and quantitative image analysis.

Arivis Pro for AI-assisted segmentation, analysis of large image datasets, multidimensional visualization, and automated workflows.

Advanced Microscopy

Confocal laser scanning microscopy and improved-resolution imaging of subcellular structures with Airy Scan hardware. Multi-channel fluorescence imaging, Spectral imaging and spectral unmixing

Widefield fluorescence microscopy - Brightfield, phase-contrast, and DIC imaging, Tile scan - large-area imaging.
Time-lapse imaging - long-term live-cell imaging under controlled temperature and CO₂ conditions with automated multilocation and multichannel imaging.

Image Processing

Image deconvolution, noise reduction and AI-assisted denoising, spectral unmixing,3D reconstruction and rendering, image stitching, movie generation and image annotation

Quantitative Image Analysis

2D, 3D, and 4D image visualization, cell segmentation and object detection, colocalization analysis, fluorescence intensity measurements, surface, volume, and morphology quantification, AI-assisted image segmentation and analysis.

Functional Imaging

• Fluorescence Recovery After Photobleaching (FRAP) / FRET (Förster Resonance Energy Transfer)
• Cell migration and wound-healing assays
• Chemotaxis and proliferation studies
• Confluence and viability analysis using Incucyte

User Training and Technical Support

• Instrument operation training
• Experimental planning and consultation
• Assistance in optimizing imaging protocols for diverse biological samples
• Selection of appropriate imaging techniques
• Assistance with image acquisition and optimization
• Image analysis guidance
• Troubleshooting and technical support

Data Management

We are happy to assist you with your microscopy and image analysis requirements. Feel free to contact us by email or phone for any queries related to the facility or our services.

In-house Certified Users

Certified in-house users can access the facility independently after regular working hours, as well as on weekends and public holidays, subject to facility guidelines and instrument availability.

External Users

Researchers from external institutes are welcome to use the facility. Please email us with a brief description of your project, your imaging requirements, and the microscope(s) you wish to use. Our team will guide you in selecting the most suitable system and assist with scheduling your experiments.

2026

• Mehta D, Paradkar A, Rekhi B, Nayak P, Mohanty B, Chaudhari P, Gera P, Rane S, Chaturvedi P, Waghmare SK. Histone methylation defines c-Jun/Sox2/Hif1α axis that controls stemness and tumor progression in squamous cell carcinoma. Nature Communications. PMID: 42045185
• Dey P, Bishnu A, Patel J, Mishra S, Gadewal N, Ray P, Gupta S, De A. Hotspot mutations in HER2 interfaces destabilize structure, causing breast cancer treatment failure. Oncogene. 45(3):414-430. PMID: 41392272
• Mehrotra M, Shenoy P, Sakpal A, Chakraborty S, Ray P. p53-R248W evades doxorubicin mediated cell death through collective alteration in TOP2A and CYP1A1 genes in cancer cells. Biochemical and Biophysical Research Communications. 819: 153759. PMID: 42019355
• Manasi Nagare, Monalisa Sahoo, Manju Sengar, Sachin Punatar, Navin Khattry, Anant Gokarn, Bhausaheb Bagal, Hasmukh Jain, Sumeet Mirgh, Sridhar Epari, Tanuja Shet, Trupti Pradhan, Shweta Shirsat, Madan Barkume, Caroline Mathen, Poonam Gera, Rohit Kumar Verma, Elveera Saldanha, Pratik Chandrani, Jitendra Gawde, Shubhada Chiplunkar & Jyoti Kode. Defining alteration in bone marrow mesenchymal stem cells (MSC) from acute myeloid leukemia and exploring cultured MSC-conditioned media as a novel anti-leukemia therapy agent. Cancer Immunology Immunotherapy: 2026 Feb 23;75(3):83

2025

• Anthony C, Harish M, Christie J, Venkatraman P (2025). Novel interaction with PSMD9 regulates DNAJA1 turnover and mitochondrial polarity, Biochemical and Biophysical Research Communications.
• Lonare A, Raychaudhuri K, Shah S, Madhu G, Sachdeva A, Basu S, Thorat R, Gupta S, Dalal SN (2025).14-3-3σ restricts YY1 to the cytoplasm, promoting therapy resistance, and tumor progression in colorectal cancer. International Journal of Cancer.
• Jog E, Jainarayanan AK, La Ferlita A, Chakraborty A, Dalwai A, Yahya S, Shivashankar A, Choudhary BS, Chandramouli A, Kazi M, Jain D, Khapare N, B A, Khan BK, Gera P, Patil P, Thorat R, Verma N, Sehgal L, Saklani A, Kamat SS, Dalal SN, Chaudhary N. Inhibiting de novo lipogenesis identifies a therapeutic vulnerability in therapy-resistant colorectal cancer. Redox Biol. 2025 Feb;79:103458. Epub 2024 Dec 11. PMID: 39705849; PMCID: PMC11729006.
• Choudhary BS, Chaudhary N, Khan BK, Vijan A, Mandal D, Pilankar L, Gawand S, Uttankar P, Sharma M, Shivashankar A, Doloi R, Joshi N, Das M, Dalal SN. LCN2 promotes focal adhesion formation and invasion by stimulating c-Src activation. Journal of Cell Science. 138(11): jcs263663. PMID: 40356520
• Jaiswal MA, Karn A, Das A, Kumari A, Tiwari S, Dalal SN. 14-3-3ε inhibits premature centriole disengagement by inhibiting the activity of Plk1 and separase. Journal of Cell Science. 138(14): jcs263808PMID: 40576425
• Chaudhary N, Mandal D, Choudhary B, Patra S, Jain D, Poonia P, Shaikh S, Tekalkar S, Malvankar S, Shivashankar A, Jog E, Pilankar L, Thorat R, Kailje V, Khanna S, Manna S, Khan B, Jadhav A, Sharma K, Ramu S, Sahoo S, Jolly MK, Dalal SN, Sinha S, Nair N, Ruppin E, Lamballe F, Maina F, Verma N. Low-GPX4 drives a sustained drug-tolerant persister state in TNBC by a targetable adaptive FSP1 upregulation. Redox Biology.
• Joshi P, Bane S, Chaturvedi P, Gera P, Waghmare SK (2025).Establishment and characterization of patient-derived tongue squamous cell carcinoma cell lines. Human Cell. 38(4):102.
• Mehta D, Paradkar A, Nayak P, Rekhi B, Mohanty B, Chaudhari P, Waghmare SK. Establishment and molecular characterization of the novel cutaneous squamous cell carcinoma cell line from advanced-stage Indian patient. Human Cell.38(4):108. PMID: 40413685
• Shenoy PS, Dash S, Joshi D, Rekhi B, Gota V, Ray P. Withaferin A Exerts Cytotoxicity in Single/multidrug-resistant gastric and ovarian cancer cells and tumor xenografts through the AKT-NF-κB-STAT3-survivin axis. Molecular Carcinogenesis
• Chaudhary N, Choudhary BS, Shivashankar A, Manna S, Ved K, Shaikh S, Khanna S, Baar J, Dani J, Sahoo S, Soundharya R, Jolly MK, Verma N. EGFR-to-Src family tyrosine kinase switching in proliferating-DTP TNBC cells creates a hyperphosphorylation-dependent vulnerability to EGFR TKI. Cancer Cell International. 25(1):55. PMID: 39972345.
• Ghosh K, Iyer RK, Sood S, Islam MS, Labad JG, Khadilkar RJ. Genetic perturbation of cellular homeostasis regulates integrated stress response signaling to control Drosophila hematopoiesis. Biology Open. 14(7): bio062046. PMID: 40554752
• Tejashree Mahaddalkar, Archisman Banerjee, Madhura Ketkar, Rahul Thorat, Nilesh Gardi & Shilpee Dutt. Oncogene, volume 44, pages2103–2115 April (2025). Aurora Kinase A and B inhibition abrogates ‘Neosis’, a non-mitotic cell division of GBM residual cells and prevents GBM recurrence.
• Chaudhary N, La Ferlita A, Choudhary BS, Jog E, Kazi M, Yahya S, Dalwai A, Ostwal V, Kumar S, Redkar S, Khapare N, Kailaje V, B A, Gera P, Bal M, Verma N, Thorat R, Saklani A, Sehgal L, Dalal SN (2025). Patient-derived Organoids and Xenografts Uncover Therapeutic Vulnerabilities in Colorectal Signet Ring Cell Carcinomas. Clinical Cancer Research

2024

• Choudhary BS, Chaudhary N, Vijan A, Mandal D, Pilankar L, Gawand S, Khan B, Shivashankar A, Doloi R, Joshi N, Das M, Dalal SN (2024). LCN2 promotes focal adhesion formation and invasion by stimulating Src activation. bioRxiv.
• Tilwani S, Gandhi K, Dalal SN (2024). 14-3-3ε conditional knockout mice exhibit defects in the development of the epidermis. FEBS Letters.
• Roy S, Mehta D, Paradkar A, Chovatiya G, Waghmare SK (2024). Dab2 (Disabled-2), an adaptor protein, regulates self-renewal of hair follicle stem cells. Communications Biology. 7(1): 525.
• Roy S, Mehta D, Sawant S, Waghmare SK (2024). Disabled 2 (Dab2) Regulates Tumour Progression in Skin Squamous Cell Carcinoma
• Phadte P, Bishnu A, Dey P, M M, Mehrotra M, Singh P, Chakrabarty S, Majumdar R, Rekhi B, Patra M, De A, Ray P (2024). Autophagy-mediated ID1 turnover dictates chemo-resistant fate in ovarian cancer stem cells. Journal of Experimental and Clinical Cancer Research. 43(1):222

2023

• Christie J, Anthony CM, Harish M, Mudartha D, Farooqee SBUD, Venkatraman P. The interaction network of the proteasome assembly chaperone PSMD9 regulates proteostasis. FEBS Journal.
• Choudhary B, Chaudhary N, Shah M, Dwivedi N, Smitha PK, Das M, Dalal SN. Lipocalin 2 inhibits actin glutathionylation to promote invasion and migration. FEBS Letters.
• Pratiksha Mahadik, Sejal Patwardhan. ECM stiffness-regulated exosomal thrombospondin-1 promotes tunneling nanotubes-based cellular networking in breast cancer cellsArchives of Biochemistry and Biophysics, 742, 109624, (2023) PMID: 37146866
• Joshi P, Waghmare S. Molecular signaling in cancer stem cells of tongue squamous cell carcinoma: Therapeutic implications and challenges. World Journal of Stem Cells 15(5): 438-452.
• Mehta D, Shaikh S, Mohanty B, Chaudhari P, Waghmare SK (2023). Secretory phospholipase (sPLA2-IIA) regulates breast cancer stem cells differentiation and metastatic potential. Biochemical and Biophysical Research Communication. 677:98-104. PMID: 37566923.
• Navarange SS, Bane SM, Mehta D, Shah S, Gupta S, Waghmare SK (2023). Epithelial-to-mesenchymal transition status correlated with ultrastructural features, and TP53 mutation in patient-derived oral cancer cell lines. Molecular Biology Reports. 50(10):8469-8481. PMID: 37639153
• Nazia Chaudhary, Bhagya Shree Choudhary, Anusha Shivashankar, Subhakankha Manna, Khyati Ved, Shagufa Shaikh, Sonal Khanna, Jeetnet Barr, Jagruti Dani, Nandini Verma. EGFR-to-Src family tyrosine kinase switching in proliferating-DTP TNBC cells creates a hyperphosphorylation-dependent vulnerability to EGFR TKI. bioRxiv 2023.07.17.549374;
• Kode, Jyoti; Maharana, Jitendra; Dar, Asif; Mukherjee, Shayanti; Gadewal, Nikhil; Sigalapalli , Dilep; Kumar, Satyanshu; Panda, Debashis; Ghosh , Soumyajit ; Keshry , Supriya ; Mamidi , Prabhudutta ; Chattopadhyay , Soma ; Pradhan, Trupti; Kailaje, Vaishali; Inamdar, Sunil; Gujjarwar , Vidula. 6-Shogaol exhibits anti-viral and anti-inflammatory activity in COVID-19 associated inflammation by regulating NLRP3 inflammasome. ACS Omega 8, 2, 2618–2628
• Low levels of CD26 on certain cellular subtypes of donor harvest is associated with better clinical outcomes post allogeneic stem cell transplantation through regulation of NF-κB pathway and pro-inflammatory cytokines. International Immunopharmacology. 125(Part A): 111054.PMID: 37890379. Kandekar S, Punatar S, Khattry N Gokarn A, Jindal N, Mirgh S, Chichra A, Tembhare P, Rane P, Gawade J, Mathew L, Patil A, Chiplunkar S, Kode J (2023).
• RajeshKumar Meher , Jitendra Maharana , Jeshma Kovvuri , K.Nirmal Kumar , Trupti Pradhan , Vaishali Kailaje , Arvind Ingle , Subrata Sen , Madan Barkume, Meena Patkar, Anand Vaibhaw, ShowkatAhmad Mir, Arpita Ghosh, Sanjeev Kamte, Ahmed Kamal j, Jyoti Kode. A Phenstatin-based indole-chalcone hybrid as novel anti-inflammatory agent targeting the NLRP3 Inflammasome: Leads from computational and preclinical studies
• Dey P, Gadewal N, De A. Pathogenic HER3 dimerization domain mutations create a structural bias towards un-conventional EGFR-HER3 signalling axis in breast cancer. International Journal of Biological Macromolecules.
• Mehta D, Shaikh S, Mohanty B, Chaudhari P, Waghmare SK (2023). Secretory phospholipase (sPLA2-IIA) regulates breast cancer stem cells differentiation and metastatic potential. Biochemical and Biophysical Research Communication. 677:98-104. PMID: 37566923.

2022

• Mal A, Bukhari AB, Singh RK, Kapoor A, Barai A, Deshpande I, Wadasadawala T, Ray P, Sen S, De A. EpCAM-Mediated Cellular Plasticity Promotes Radiation Resistance and Metastasis in Breast Cancer. Frontiers in Cell Developmental Biology. 8:597673 2021 PMID: 33490064
• Bose, A., Modi, K., Dey, S., Dalvi, S., Nadkarni, P. Sudarshan, M., Tapas K. Kundu, T.K., Venkatraman, P., and Dalal, S.N. 14-3-3γ prevents centrosome duplication by inhibiting NPM1 function. Genes to Cells, 26, 426-446 2021).
• Sarika Tilwani, Karan Gandhi, Satya Narayan, Sri Rama Koti Ainavarapu, Sorab Nariman Dalal (2021).Disruption of desmosome function leads to increased centrosome clustering in 14-3-3γ-knockout cells with supernumerary centrosomes.
• Raghavan, R., Koyand, N., Beher, R., Chetlangia, N., Ramadwar, M., Pawade, S., Thorat, R., van Hengel, J., Sklyarova, T., van Roy, F., and Dalal, S.N. (2022). Plakophilin3 loss leads to increased adenoma formation and rectal prolapse in APCmin mice. Biochem. Biophys. Res. Commun.586, 14-19
• N. Chaudhary, N. Joshi, R. Doloi, A. Shivashankar, R. Thorat and S. N. Dalal. (2022). Plakophilin3 loss leads to an increase in autophagy and radio-resistance. Biochem Biophys Res Commun.620, 1-7.
• Singh, Bhawna, et al. The BRCA2 and CDKN1A interacting protein (BCCIP) stabilizes stalled replication forks and prevents degradation of nascent DNA." FEBS letters 596.16 (2022): 2041-2055
• Mulla SW, Venkatraman P. Novel Nexus with NFκB, β-catenin, and RB1 empowers PSMD10/Gankyrin to counteract TNF-α induced apoptosis establishing its oncogenic role. International Journal of Biochemistry & Cell Biology. 146: 106209. PMID: 35378311.
• Mukherjee S, Sakpal A, Mehrotra M, Phadte P, Rekhi B, Ray P (2022). Homo and heterotypic cellular cross-talk in epithelial ovarian cancer impart pro-tumorigenic properties through differential activation of the Notch3 pathway. Cancers. 14(14): 3365.
• Mishra SK, Dhadve AC, Mal A, Reddy BPK, Hole A, CM Krishna, Ray P, Srivastava R, De A. Photothermal therapy (PTT) is an effective treatment measure against solid tumors which fails to respond conventional chemo/radiation therapies in clinic. Biomaterials Advances. 143:213153. PMID: 36343390
• Mukherjee S, Sharma S, Soni V, Joshi A, Gaikwad A, Bellare J, Kode J. Improved osteoblast function on titanium implant surfaces coated with nanocomposite Apatite-Wollastonite-Chitosan- an experimental in-vitro study. Journal of Materials Science: Materials in Medicine. 33(3):25. PMID: 35190908.

2021

• Sheikh Burhan Ud Din Farooqee, Joel Christie, Prasanna Venkatraman. PSMD9 ribosomal protein network maintains nucleolar architecture and WT p53 levels, Biochemical and Biophysical Research Communications, 2021
• Chaudhary N, Choudhary BS, Shah SG, Khapare N, Dwivedi N, Gaikwad A, Joshi N, Raichanna J, Basu S, Gurjar M, Smitha PK, Saklani A, Gera P, Ramadwar M, Patil P, Thorat R, Gota V, Dhar SK, Gupta S, Das M, Dalal SN. Lipocalin 2 expression promotes tumor progression and therapy resistance by inhibiting ferroptosis in colorectal cancer, Int J Cancer.
• Bose A, Modi K, Dey S, Dalvi S, Nadkarni P, Sudarshan M, Kundu TK, Venkatraman P, Dalal SN. 14-3-3γ prevents centrosome duplication by inhibiting NPM1 function. Genes Cells, 26(6):426-446. PMID: 33813791
• Mal A, Bukhari AB, Singh RK, Kapoor A, Barai A, Deshpande I, Wadasadawala T, Ray P, Sen S, De A. EpCAM-Mediated Cellular Plasticity Promotes Radiation Resistance and Metastasis in Breast Cancer. Frontiers in Cell Developmental Biology. 8:597673 2021 PMID: 33490064

2020

• Ramchandra V Amnekar, Shafqat A Khan, Mudasir Rashid, Bharat Khade, Rahul Thorat, Poonam Gera, Shailesh V Shrikhande, Duane T Smoot, Hassan Ashktorab, Sanjay Gupta. Histone deacetylase inhibitor pre-treatment enhances the efficacy of DNA-interacting chemotherapeutic drugs in gastric cancer (2020). World J Gastroenterol 2020 February 14; 26(6): 598-613
• Sanket G. Shah, Tripti Verma, Mudasir Rashid, Nikhil Gadewal, Sanjay Gupta. Histone H2A isoforms: Potential implications in epigenome plasticity and diseases in eukaryotes (2020). J of Biosciences 45:4
• Asmita Sharda, Mudasir Rashid, Sanket Shah, Ajit Kumar Sharma, Saurav Raj Singh, Poonam Gera, Murali Krishna Chilakapati, Sanjay Gupta. Elevated HDAC activity and altered histone phospho-acetylation confer acquired radio-resistant phenotype to breast cancer cells (2020) Clinical Epigenetics. 12:4
• Sanket G. Shah, Tushar Mandloi, Pooja Kunte, Abhiram Natu, Mudasir Rashid, Divya Reddy, Nikhil Gadewal & Sanjay Gupta. HISTome2: a database of histone proteins, modifiers for multiple organisms and epidrugs. Epigenetics & Chromatin; 13: 31 (2020)
• Mukherjee S, Agarwal M, Bakshi A, Sawant S, Thomas L, Fujii N, Nair P, Kode J. Chemokine SDF1 Mediated Bone Regeneration Using Biodegradable Poly(D, L-lactide-co-glycolide) 3D Scaffolds and Bone Marrow-Derived Mesenchymal Stem Cells: Implication for the Development of an “Off-the-Shelf” Pharmacologically Active Construct. Biomacromolecules
• Kode J, Kovvuri J, Nagaraju B, Jadhav S, Barkume M, Sen S, Kasinathan NK, Chaudhari P, Mohanty BS, Gour J, Sigalapalli DK, Kumar CG, Pradhan T, Banerjee M, Kamal A. Synthesis, biological evaluation, and molecular docking analysis of phenstatin based indole linked chalcones as anticancer agents and tubulin polymerization inhibitors. Bioorganic Chemistry.
• Dimri S, Malhotra R, Shet T, Mokal S, Gupta S, De A (2020). Noncanonical pS727 post translational modification dictates major STAT3 activation and downstream functions in breast cancer. Experimental Cell Research. 396(2): 112313. PMID: 33002501
• Rathod M, Kelkar M, Valvi S, Salve G, De A (2020). FOXA1 regulation turns benzamide HDACi treatment effect specific in breast cancer promoting NIS gene mediated targeted radioiodine therapy. Molecular Therapy: Oncolytics. 19: 93-104. PMID: 33102692
• Mal A, Dey P, Hayes RM, McCarthy JV, Ray A, De A (2020). In Silico Identification of Potential Phosphorylation in the Cytoplasmic Domain of Epithelial Cell Adhesion Molecule. ACS Omega. 5(48): 30808-30816. PMID: 33324790
• Ghorai, A., Mahaddalkar, T., Thorat, R. and Dutt, S. (2020). Sustained inhibition of PARP-1 activity delays glioblastoma recurrence by enhancing radiation-induced senescence. Cancer Lett. 490, 44-53.

2019

• Harish M, Kannan S, Puttagunta S, Pradhan MR, Verma CS, Venkatraman P. A Novel Determinant of PSMD9 PDZ Binding Guides the Evolution of the First Generation of Super Binding Peptides. Biochemistry. 58(32): 3422-3433, 2019. PMID: 31287951.
• Sahu I, Nanaware P, Mane M, Mulla SW, Roy S, Venkatraman P. Role of a 19S Proteasome Subunit- PSMD10(Gankyrin) in Neurogenesis of Human Neural Progenitor Cells. Int J Stem Cells. 12(3):463-473, 2019. PMID: 31474027

2018

• Mansa Gurjar, Kumar Krishna Raychaudhuri, Snehal Mahadika, Divya Reddy, Apurva Atak, Trupti Shetty Kruthi Rao, Mansi S. Karkhanis, Prajakta Gosavi, Lalit Sehgal, Sanjay Gupta, Sorab N. Dalal. Plakophilin3 increases desmosome assembly, size and stability by increasing expression of desmocollin2, Biochemical and Biophysical Research Communications, Volume 495, Issue 1, 1 January 2018, Pages 768-774
• Vishal SS, Tilwani S, Dalal SN. Plakoglobin localization to the cell border restores desmosome function in cells lacking 14-3-3γ. Biochem Biophys Res Commun. 495(2): 1998-2003. PMID: 29253567
• Basu S, Chaudhary N, Shah S, Braggs C, Sawant A, Vaz S, Thorat R, Gupta S, Dalal SN. Plakophilin3 loss leads to an increase in lipocalin2 expression, which is required for tumour formation. Exp Cell Res. 369(2): 251-265. PMID: 29803740

2017

• Kelkar MG, Thakur B, Derle A, Chatterjee S, Ray P, De A. Tumor suppressor protein p53 exerts negative transcriptional regulation on human sodium iodide symporter gene expression in breast cancer. Breast Cancer Res Treat. 164(3), 603-615.
• Chovatiya, G.L., Sarate, R.M., Sunkara, R.R., Gawas, N.P., Kala, V., and Waghmare, S.K. Secretory phospholipase A2-IIA overexpressing mice exhibit cyclic alopecia mediated through aberrant hair shaft differentiation and impaired wound healing response. Sci Rep 7, 11830-9, PMID: 28912581
• Rathod M, Chatterjee S, Dutta S, Kalraiya R, Bhattacharya D, De A. Mannose glycosylation is an integral step for human NIS localization and function in breast cancer cells (2019). Journal of Cell Science. PMID: 31455607.

2016

• Mukhopadhyay, A., Sehgal, L., Bose, A., Gulvady, A., Senapati, P., Thorat, R., Basu, S., Bhatt, K., Hosing, A. S., Balyan, R., Borde, L., Kundu, T. K., and Dalal, S. N. 2016. 14-3-3γ prevents centrosome amplification and neoplastic progression. Scientific Reports 6, Article number: 26580 (2016)
• Raychaudhuri, K., Chaudhary, N., Gurjar, M., D'Souza, R., Limzerwala, J., Maddika, S., and Dalal, S. N. (2016). 14-3-3sigma loss leads to activation of the epithelial to mesenchymal transition due to the stabilization of c-Jun. J Biol Chem 291: 16068-16081. (2016).
• Rahul M. Sarate, Gopal L. Chovatiya, Vagisha Ravi, Bharat Khade, Sanjay Gupta, Sanjeev K. Waghmare. sPLA2 -IIA Overexpression in Mice Epidermis Depletes Hair Follicle Stem Cells and Induce Differentiation Mediated through Enhanced JNK/c-Jun Activation. Stem Cells, 34(9):2407-17, 2016

2015

• Chatterjee S, Thaker N, De A. Combined 2-deoxy glucose and metformin improves therapeutic efficacy of sodium-iodide symporter-mediated targeted radioiodine therapy in breast cancer cells. Breast Cancer (Dove Med Press). 7:251-65.
• Basu S, Thorat R, Dalal SN. MMP7 is required to mediate cell invasion and tumor formation upon plakophilin3 loss.
• Shafqat A. Khan, Divya R Velga, Sanjay Gupta. Global Histone Posttranslational Modifications and Cancer: Biomarkers for Diagnosis, Treatment and Prognosis? World Journal of Biological Chemistry, 6(4): 333–345, 2015
• Ajit K. Sharma, Shafqat A. Khan, Asmita Sharda, Divya R Velga, Sanjay Gupta. MKP1 phosphatase mediates G1-specific dephosphorylation of H3Serine10P in response to DNA damage Mutation Research, 778:71-9, 2015
• Ajit K. Sharma, Saikat Bhattacharya, Shafqat A. Khan, Bharat Khade, Sanjay Gupta. Dynamic alteration in H3 serine 10 phosphorylation is G1-phasespecific during ionization radiation induced DNA damage response inhuman cells. Mutation Research, 773: 83–91
• Snehal M.Gaikwad ,BhushanThakur, Asmita Sakpal, Ram K.Singh Pritha Ray. Differential activation of NF-κB signaling is associated with platinum and taxane resistance in MyD88 deficient epithelial ovarian cancer cells

2014

• Sehgal L, Usmani, A. Dalal SN, Majumdar, SS. Generation of transgenic mice by exploiting spermatogonial stem cells in vivo. Methods Mol Biol 1194: 327-337.
• Sehgal L, Mukhopadhyay A, Rajan A, Khapare N, Sawant M, Vishal SS, Bhatt K, Ambatipudi S, Antao N, Alam H, Gurjar M, Basu S, Mathur R, Borde L, Hosing AS, Vaidya MM, Thorat R, Samaniego F, Kolthur-Seetharam U, Dalal SN. 14-3-3gamma mediated transport of plakoglobin to the cell border is required for the initiation of desmosome assembly in vitro and in vivo. J Cell Sci 127: 2174-2188
• Shafqat A Khan, Monica Tyagi, Ajit K Sharma, Savio G Barreto, Bhawna Sirohi, Mukta Ramadwar, Shailesh V Shrikhande, Sanjay Gupta. Cell-type specificity of β-actin expression and its clinicopathological correlation in gastric adenocarcinoma. World Journal of Gastroenterology, 20(34):12202-11. 2014
• Ram K.Singha, Snehal M.Gaikwada, Ankit Jinagera, Smrita Chaudhurya, Amita Maheshwari, Pritha Ray. IGF-1R inhibition potentiates cytotoxic effects of chemotherapeutic agents in early stages of chemoresistant ovarian cancer cells

2013

• Iyer SV, Dange PP, Alam H, Sawant SS, Ingle AD, Borges AM, Shirsat NV, Dalal SN, Vaidya MM. Understanding the role of keratins 8 and 18 in neoplastic potential of breast cancer derived cell lines. PLoS One 8: e53532.
• Chatterjee S, Malhotra R, Varghese F, Bukhari AB, Patil A, Budrukkar A, Parmar V, Gupta S, De A. Quantitative Immunohistochemical Analysis Reveals Association between Sodium Iodide Symporter and Estrogen Receptor Expression in Breast Cancer. PLoS One. 8(1): e54055. [IF 3.53]

2012

• Alam H, Bhate AV, Gangadaran P, Sawant SS, Salot S, Sehgal L, Dange PP, Chaukar DA, D'Cruz A K, Kannan S, Gude R, Kane S, Dalal SN, Vaidya MM. Fascin overexpression promotes neoplastic progression in Oral Squamous Cell Carcinoma. BMC Cancer 12:32.
• Khapare N, Kundu ST, Sehgal L, Sawant M, Priya R, Gosavi P, Gupta N, Alam H, Karkhanis M, Naik N, Vaidya MM, Dalal SN. Plakophilin3 Loss Leads to an Increase in PRL3 Levels Promoting K8 Dephosphorylation, Which Is Required for Transformation and Metastasis. PLoS One 7: e38561.

2011

• Gosavi P, Kundu ST, Khapare N, Sehgal L, Karkhanis MS, Dalal SN. E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly. Cell Mol Life Sci 68:1439-1454.
• Sehgal L, Thorat R, Khapare N, Mukhopadhaya A, Sawant M, Dalal SN. Lentiviral mediated transgenesis by in vivo manipulation of spermatogonial stem cells. PLoS One 6: e21975.
• Telles E, Gurjar M, Ganti K, Gupta D, Dalal SN. Filamin A stimulates cdc25C function and promotes entry into mitosis. Cell Cycle 10:776-782.
• Alam H, Kundu ST, Dalal SN, Vaidya MM. Loss of keratins 8 and 18 leads to alterations in alpha6beta4-integrin-mediated signalling and decreased neoplastic progression in an oral-tumour-derived cell line. J Cell Sci 124:2096-2106.
• Alam H, Sehgal L, Kundu ST, Dalal SN, Vaidya MM. Novel function of keratins 5 and 14 in proliferation and differentiation of stratified epithelial cells. Mol Biol Cell 22:4068-4078.

2009

• Telles E, Hosing AS, Kundu ST, Venkatraman P, Dalal SN. A novel pocket in 14-3-3epsilon is required to mediate specific complex formation with cdc25C and to inhibit cell cycle progression upon activation of checkpoint pathways. Exp Cell Res 315:1448-1457.

2008

• Hosing AS, Kundu ST, Dalal SN. 14-3-3 Gamma is required to enforce both the incomplete S phase and G2 DNA damage checkpoints. Cell Cycle 7:3171-3179.
• Kundu ST, Gosavi P, Khapare N, Patel R, Hosing AS, Maru GB, Ingle A, Decaprio JA, Dalal SN. Plakophilin3 down regulation leads to a decrease in cell adhesion and promotes metastasis. Int J Cancer 123:2303-2314.
• Garg R, Gupta S and Maru G B. Dietary curcumin modulates transcriptional regulators of phase I and phase II enzymes in benzo[a]pyrene-treated mice: mechanism of its anti-initiating action. Carcinogenesis. 29(5):1022-1032

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Facility Email:adif@actrec.gov.in
Mrs. Vaishali Kailaje: vaishali.kailaje@actrec.gov.in
Mrs. Tanuja Durve: tanuja_d29@gmail.com
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