Dynamic modulation of key kinases govern development of chemo resistance through multiple mechanisms. Currently we are interested in understanding how MAPK/ERK and PI3K/AKT signalling fine tune autophagic flux to promote development of platinum-taxol dual resistance. Further we are developing state of art optical sensors for real time non-invasive imaging of these key signalling pathways in order to delineate and implement an optimal therapeutic strategy at precise therapeutic window to combat chemo resistance.
Protein-protein interactions are the fingerprints of variety of biological phenomena and provide critical inputs about molecular response of epithelial ovarian cancer cells to chemotherapy. We developed Bioluminescence resonance energy transfer (BRET) based sensor to monitor therapy induced PIP3/AKT and ERK1/2 activation in real-time. We are currently applying our BRET sensor platform to evaluate the efficacy of non-platinum drugs in cancer cells derived from malignant ascites and chemoresistant ovarian cancer cells.
Senior Research Fellow
Aniketh Bishnu
Ray Lab