Electron Microscopy
The Electron Microscope Facility at ACTREC is equipped with JEM 1400 PLUS, a Transmission electron microscope (JEOL, Japan) functioning at 120kV with magnification up to 12,00,000 X and 0.2nm resolution. The facility provides services for research and analysis of biological samples, nanoparticles and other applications.
Electron Microscopy ATLAS
- The facility carries out sample preparation for routine electron microscopy, including resin block making (solid tissues, monolayer cell cultures, and cell suspensions) followed by ultrathin sectioning using Leica UC-7 ultramicrotome, contrasting, and acquisition.
- The facility also carries out negative staining for small particles (<100nm) like bacteriophages, exosomes, nanoparticles, proteins and DNA.
- The facility provides acquisition of immunogold labelled macromolecules to detect specific localization.
- The facility provides elemental detection by Bruker EDS system
The major areas of electron microscopy analysis are,
- Ultrastructure study of cell and tissue,
- Studying the details at organelle level like mitochondrial changes, ER - mitochondrial interactions, mitochondrial cristae, cell membrane, nuclear architecture, golgi complexes etc.,
- To check events of autophagy, apoptosis or necroptosis,
- Characterization of exosomes, bacteriophages and nanoparticles,
- Uptake and effect of nanoparticles / drugs / radiation on cells or tissue,
- EDS analysis of nanoparticles
Please write to the facility regarding your requirement for electron microscopy. The facility will provide the details as per the analysis requirement. The users are requested to discuss over email regarding their study and sample submission. The samples need to be submitted along with a duly filled requisition form. The requisition form will be provided by the facility on request.
ACTREC & DAE | Other than ACTREC institutions | Corporate | ||
---|---|---|---|---|
Routine EM | 1000 | 5000 | 10000 | |
Routine EM + Immunogold labeling | 1500 | 6000 | 12000 | |
Negative staining | 800 | 4000 | 8000 |
18% GST to be included in addition to the following charges.
Mode of Payment
The charges should be paid against the quotation or invoice to the following account. The epayment details will be provided by the facility on request. The transaction details should be sent by email to the facility. The acquisition will be carried out after the receipt of the payment is confirmed.
Facility-in-charge: Siddhi A. Redkar
Technical Officer ‘D’
Electron Microscope Facility
(Khanolkar Shodhika - 68)
ACTREC, TMC
Plot No.1 & 2, Sector 22
Kharghar, Navi Mumbai - 410 210.
022-27405000 / 022-68735000
Extension : 5545
Email : tem.facility@actrec.gov.in
EM Staff: Ms. Arpana Kadam, Scientific Assistant ‘C’
Mr. Shridhar Nadkar, Technician ‘F’
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Cell to cell Contact | Cell |
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Exosomes | Mitochondria |
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Nanoparticles | Golgi Complex |
2025
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- Nazia Choudhary et. al. Inhibiting de novo lipogenesis identifies a therapeutic vulnerability in therapy-resistant colorectal cancer. Redox Biology. Feb. 2025
- Narasimha Anaganti, Aman Kumar Ujaoney, Biochemical characterization and functional insights into DNA substrate-specific activities of a unique radiation-inducible DR1143 protein from Deinococcus radiodurans, International Journal of Biological Macromolecules 310 (April 2025) 143214
- Roma Dohara, Dibyendu Bhattacharyya (2025). Intra-Golgi Golgin PpSgm1 and GRIP domain Golgin PpImh1 synergistically mediate Golgi cisternal stacking. Journal of Cell Science. PMID: 263612. April 2025
- Priyanka Joshi, Sanjeev K. Waghmare, et. al., Establishment and characterization of patient derived tongue squamous cell carcinoma cell lines. Human Cell (2025) 38:102
- Darshan Mehta, Sanjeev K Waghmare, et.al, Establishment and molecular characterization of the novel cutaneous squamous cell carcinoma cell line from advanced stage Indian patient. Human Cell (May 2025) 38:108
- Monika A. Jaiswal, Sorab N. Dalal, et.al, 14-3-3ε inhibits premature centriole disengagement by inhibiting the activity of Plk1 and Separase. Journal of Cell Science (2025) June 2025
- Chetna, De Lab, Nanoscale
2024
- Megha Garg et.al., Implications of chronic moderate protein-deficiency malnutrition on doxorubicin pharmacokinetics and cardiotoxicity in early post-weaning stage, Life Sciences 350 (2024) 122765
- Vandan Nagar et.al. Characterization and Genome Analyses of the Novel Phages P2 and vB_AhydM-H1 Targeting Aeromonas hydrophila, PHAGE,
- Madhura Ketkar et al, Inhibition of PERK mediated UPR acts as a switch for reversal of residual senescence and as senolytic therapy in glioblastoma, Neuro-oncology, doi/10.1093/neuonc/noae134/7716143, 22 July 2024
- Pratham Phadte, et.al. Autophagy-mediated ID1 turnover dictates chemo-resistant fate in ovarian cancer stem cells. J Exp Clin Cancer Res (2024) 43:222
- Amol Lonare, Sorab N Dalal, 14-3-3σ restricts YY1 to the cytoplasm, promoting therapy resistance, and tumor progression in colorectal cancer, Int J Cancer, 2024 Sep 6
- Radha G., Manu Lopus et.al., Biochemical and in silico analysis of the binding mode of erastin with tubulin. Journal of biomolecular structure and dynamics
- Kimaya Meher, Manu Lopus, et.al., Induction of autophagy-dependent and caspase and microtubule-acetylation-independent cell death by phytochemical-stabilized gold nanopolygons in colorectal adenocarcinoma cells. The Royal Society of Chemistry, Nanoscale,2024,16,7976–7987
2023
- Shreosi Chatterjee, Abira Ganguly, Dibyendu Bhattacharyya. Reprogramming nucleolar size by genetic perturbation of the extranuclear Rab GTPases Ypt6 and Ypt32. FEBS Letters 2023, 598(2024) 283–301
- Aman Kumar Ujaoney, Narasimha Anaganti, Mahesh Kumar Padwal, Bhakti Basu Deinococcus lineage and Rad52 family-related protein DR0041 is involved in DNA protection and compaction. International Journal of Biological Macromolecules 125885
- Sushant S Navarange 1 2, Sanjay M Bane 1, Darshan Mehta 1 2, Sanket Shah 3 2, Sanjay Gupta 3 2, Sanjeev K Waghmare 4Epithelial-to-mesenchymal transition status correlated with ultrastructural features, and TP53 mutation in patient-derived oral cancer cell lines. Mol Biol Rep. 2023 Oct: 50(10):8469-8481
- Neha Mishra et.al. Structural implications of amyloidogenic rare variants Ser282Leu and Gln356Arg identified in h-BRCA1. Proteins. 2023;–14
- Suchita Dubey, Neha Mishra, Nabajyoti Goswami, M. Quadir Siddiqui, Ashok K. Varma, Multimodal approach to characterize the tetrameric form of human PML-RBCC domain and ATO-mediated conformational changes. International Journal of Biological Macromolecules 223 (2022) 468–478.
- Sukanya Rauniyar, Kshama Pansare, Asmita Sharda, Saurav Raj Singh, Panchali Saha, Murali Krishna Chilakapati, and Sanjay Gupta. Raman Spectroscopy Revealed Cell Passage-Dependent Distinct Biochemical Alterations in Radiation-Resistant Breast Cancer Cells. ACS Omega 2023, 8, 5522−5532.
2022
- Nazia Chaudhary, et. al. Plakophilin3 loss leads to an increase in autophagy and radio-resistance. Biochemical and Biophysical Research Communications. Volume 620, 10 September 2022, Pages 1-7
- Pallavi Shukla, Prerana Dange, et.al. ARID2 suppression promotes tumor progression and upregulates cytokeratin 8, 18 and β-4 integrin expression in TP53-mutated tobacco-related oral cancer and has prognostic implications. Cancer Gene Therapy (2022)
- Dongre HN, Mahadik S, Ahire C, Rane P, Sharma S, Lukmani F, Patil A, Chaukar D, Gupta S, Sawant SS (2022). Diagnostic and prognostic role of protein and ultrastructural alterations at cell-extracellular matrix junctions in neoplastic progression of human oral malignancy. Ultrastructural Pathology.
2021
- Kakoli Bose, Ajay Wagh, et.al., Loss of GSK-3β mediated phosphorylation in HtrA2 contributes to uncontrolled cell death with Parkinsonian phenotype. International Journal of Biological Macromolecules 180 (2021) 97–111.
- Aniketh Bishnu, et.al., Molecular imaging of the kinetics of hyperactivated ERK1/2-mediated autophagy during acquirement of chemoresistance. Cell Death and Disease (2021) 12:161.
- Rajendra J, Ghorai A, Dutt S. Heliyon. 2021 Nov 12;7(11):e08371. 14-3-3ζ negatively regulates mitochondrial biogenesis in GBM residual cells.
- Prasad Sulkshane, et.al., Elevated USP9X drives early-to-late-stage oral tumorigenesis via stabilisation of anti-apoptotic MCL-1 protein and impacts outcome in oral cancers. British Journal of Cancer
- Sheikh Burhan Ud Din Farooqee, et.al. PSMD9 ribosomal protein network maintains nucleolar architecture and WT p53 levels. Biochemical and Biophysical Research Communications 563 (2021) 105-112.
2020
- Sudeshna Roy Chowdhury, et.al., ER arrival sites associate with ER exit sites to create bidirectional transport portals. J. Cell Biol. 2020 Vol. 219 No.4.
- Asmita Sharda, et.al., Elevated HDAC activity and altered histone phospho-acetylation confer acquired radioresistant phenotype to breast cancer cells. Clinical Epigenetics (2020) 12:4
- Ekjot Kaur, et.al., Inhibition of SETMAR–H3K36me2–NHEJ repair axis in residual disease cells prevents glioblastoma recurrence.Neuro-Oncology 22(12), 1785–1796, 2020
- Salunkhe S, et al., Biochim Biophys Acta Bioenerg. 2020 Dec 1;1861(12):148300. Metabolic rewiring in drug resistant cells exhibit higher OXPHOS and fatty acids as preferred major source to cellular energetics.
- Devanshi Khare, et.al., Genomic and functional insights into the adaptation and survival of Chryseobacterium sp. strain PMSZPI in uranium enriched environment. Ecotoxicology and Environmental Safety 191 (2020) 110217.
2019
- Bhawik Kumar Jain, et.al., The golgin PpImh1 mediates reversible cisternal stacking in the Golgi of the budding yeast Pichia pastoris. Journal of Cell Science (2019) 132, jcs230672
- Kshama Pansare, et.al., Establishment and genomic characterization of gingivobuccal carcinoma cell lines with smokeless tobacco associated genetic alterations and oncogenic PIK3CA mutation. Scientific Reports
2018
- Salunkhe S et al., Int J Cancer. 2018 May 15;142(10):2175-2185. Inhibition of novel GCN5-ATM axis restricts the onset of acquired drug resistance in leukemia.
- S Sawant, et.al., Alterations in desmosomal adhesion at protein and ultrastructure levels during the sequential progressive grades of human oral tumorigenesis. Eur J Oral Sci 2018; 126: 251–262. DOI: 10.1111/eos.12426.
- Nilesh Kolhe, et.al., Responses exhibited by various microbial groups relevant to uranium exposure. Biotechnology Advances 36 (2018) 1828–1846.
- Pallavi Chandwadkar, et.al., Uranium biomineralization induced by a metal tolerant Serratia strain under acid, alkaline and irradiated conditions. Metallomics, 2018,10, 1078.
- Bhawik Kumar Jain, et.al., Identification and characterization of GRIP domain Golgin a. PpImh1 from Pichia pastoris. Yeast. 2018;35:499–506
- Manjoor Ali, et.al., Thorium Decorporation Efficacy of Rationally-Selected Biocompatible Compounds with Relevance to Human Application. Journal of Hazardous Materials 2018.
- Saurabh Kirolikar, Indraneel Mittra, et al, Prevention of radiation-induced bystander effects by agents that inactivate cell-free chromatin released from irradiated dying cells. Cell Death and Disease (2018) 9:1142.
2017
- SS Sawant et al., A nomogram for predicting the risk of neck node metastasis in pathologically node-negative oral cavity carcinoma. Oral Diseases.
- Mansa Gurjar et al., Plakophilin3 increases desmosome assembly, size and stability by increasing expression of desmocollin2. Biochemical and Biophysical Research Communications (2017) 2017, 495(1):768-774.
- Chauhan DS, De A et al., 2017. NIR light triggered shrinkable thermoresponsive PNVCL nanoshells for cancer theranostics. RSC Advances, 7(70), 44026–34.
- Shanavas A, De A et al., 2017. Glycol chitosan assisted in situ reduction of gold on polymeric template for anti-cancer theranostics. International Journal of Biological Macromolecules. [Published 22-11-2017].
- Sonali S Vishal , Sarika Tilwani, Sorab N Dalal, Plakoglobin localization to the cell border restores desmosome function in cells lacking 14-3-3γ. December 2017. Biochemical and Biophysical Research Communications 495(2).
2016
- Dmello C, et al., Vimentin-mediated regulation of cell motility through modulation of beta4 integrin protein levels in oral tumor derived cells. Int. J. Biochem Cell Biol. 2016 Jan;70:161-172. Epub 2015 Nov.29.
- Sulkshane P, Teni T. BH3 mimetic Obatoclax (GX15-070) mediates mitochondrial stress predominantly via MCL-1 inhibition and induces autophagy-dependent necroptosis in human oral cancer cells. Oncotarget. Aug 5;8(36):60060-60079, 2016.
- S Sawant, et al., (2016) Establishment of 3D Co-Culture Models from Different Stages of Human Tongue Tumorigenesis: Utility in Understanding Neoplastic Progression. PLoS ONE 11(8): e0160615.
- Mukhopadhyay, A., Sehgal, L., Bose, A., Gulvady, A., Senapati, P., Thorat, R., Basu, S., Bhatt, K., Hosing, A. S., Balyan, R., Borde, L., Kundu, T. K., and Dalal, S. N. 2016 14-3-3γ prevents centrosome amplification and neoplastic progression. Scientific Reports 6, Article number: 26580 (2016)