The Flow Cytometry (FCM) facility is a centralized facility used by scientists/ clinicians from ACTREC for a wide range of research applications including Immunophenotyping, Multicolor analysis, DNA content and cell cycle analysis, Apoptosis and Proliferation studies, Detection of mitochondrial membrane potential, Stem cell analysis, Detection of circulating tumor cells, Functional assays like Intracellular calcium influx, Oxidative burst analysis, Intracellular cytokine analysis, Cytometric bead array assay for the detection of cytokines, and 4-way live cell sorting.

The facility provides technical expertise in experiment design and data interpretation to researchers whenever required, and also provides training in data analysis.

The facility has four flow cytometers: FACSAria III, FACSAria I, Attune NxT and FACSCalibur. FACSAria III is equipped with 5 lasers (UV: 355 nm, Violet: 405 nm, Blue: 488 nm, Yellow green: 561 nm and Red: 633 nm) and can perform 18 color analysis. FACSAria-I is equipped with 3 lasers (Violet: 405 nm, Blue: 488 nm and Red: 633 nm) and can analyse up to 11-color. Both the Cell sorter can perform 4-way cell sorting as well as single cell sorting into 96-well plates using the Automated Cell Deposition System. Attune NxT is equipped with 4 lasers (Violet: 405 nm, Blue: 488 nm, Yellow green: 561 nm and Red: 633 nm) and can analyse up to14 color. FACSCalibur is equipped with one laser (Blue: 488 nm) and can perform 3-color analysis.

The software used for data analysis include FlowJo, FACSDiva, CellQuest Pro, Attune NxT, FCAP Array and Modfit.

The facility also offers its services to outside investigators on payment basis. Demonstrations and training were provided to staff and students on request.

Flow cytometry facility is a centralised facility provides technical resources and professional expertise for multicolour flow cytometry analysis and cell sorting.

The following services has been provided by Facility:

• Multicolor analysis can be done efficiently up to 18 colors by FACSAria III (BD Biosciences) and 14 colors by using Attune NxT (Life Technologies).
• Cell sorting can be done by FACSAria I (up to 11 Colors and FACSAria III (up to 18 Colors). Both the cell sorters can sort 4 different populations at a time and also perform plate sorting for clonal analysis.
• Software Flow Jo, FACSDiva, ModFit (Cell cycle analysis), Attune NxT, Cell Quest Pro, FCAP Array (Cytometric Bead Array Assay analysis) can be used for analysis purpose.
• The technical support is extended to design the flow cytometry based experiments, to optimise the reagents (e.g. antibodies concentration), Florochrome selection and further assistance in data analysis.

For External User

The Flow cytometry facility at ACTREC is open to external user with certain conditions as follows:

• Booking has to be done at least one week prior in advance and depending upon the availability of the slot, booking will be available to external user.
• User should prepare the sample in their own Institute/ Lab and bring it in sterile condition.
• It is recommended to pass the sample through strainer to avoid clogging of sample line.
• The user should bear the cost of analysis of data separately.
• The data should be collected immediately after acquisition. Users are requested to carry CD to copy the data.

Charge for Outside Users

Government Institutes/ University   - Rs. 600/ Tube (for acquisition)
Corporate                  - Rs. 2000/ Tube (for acquisition)

The user should contact of any query to Flow Cytometry facility on following:

Dr. Sanjeev Waghmare
Email: swaghmare@actrec.gov.in
      flowcytometry@actrec.gov.in
Contact: 022-27405000 Ext. 5316/5122

DNA Cell Cycle using Propidium iodide

Reagents and Instruments

• Cell lines or primary cell cultures
• Phosphate buffered saline (0.01 M PBS pH 7.4)
• 70% ethanol
• Propidium iodide (PI)- Stock 400 μg/ml
• RNase- Stock 1mg/ml
• Triton X-100
• Polystyrene round bottom 12 x 75 mm Falcon tubes (Flow Tubes)
• 1 ml syringes with 26 G (Guage) needle
• Flow cytometer- FACSCalibur, Attune Nxt
• Softwares – CellQuest Pro, ModFit

Sample fixation

1. Dispense 1 x 10⁶ cells per ml of PBS in 5ml polystyrene tubes.
2. Wash the cells with 1 ml of PBS by centrifuging at 1000 rpm for 10 mins.
3. Fix the cells by adding 70% chilled ethanol drop wise to the cell pellet while vortexing. This should ensure fixation of all cells and minimize clumping.
4. Keep cells on ice for 1 hr or overnight at 4℃. (Fixed cells can be stored at 4℃ for 15-20 days)

Sample Staining

Procedure – I

1. Take 1 x 10⁶ fixed cells in 5ml polystyrene tubes and centrifuge at 1000 rpm for 10 mins.
2. Discard supernatant, wash the cells with 1 ml of PBS by centrifuging at 1000 rpm for 10 mins.
3. Add 500 μl of PBS to the cell pellet, vortex gently, add 100 μl of Propidium Iodide and 100 μl of RNase. Cover the tubes with aluminum foil to prevent its exposure to light. Incubate at 37ºC for 30 mins.
4. Pass the sample through 26 G needle to prepare single cell suspension.
5. Acquire the sample on FACSCalibur / Attune NxT.

Procedure- II

1. Take 1 x 10⁶ fixed cells in 5ml polystyrene tubes and centrifuge at 1000 rpm for 10 mins.
2. Discard the supernatant and wash the cells with 1 ml of PBS by centrifuging at 1000 rpm for 10 mins
3. Re-suspend the pellet in 500 μl of PBS containing 0.1% Triton X-100 and 100 μg/ml of RNaseA for permeabilization and RNase inactivation respectively. Incubate at 37ºC for 15 mins.
4. After incubation, add 25 μg/ml of propidium iodide to the cell suspension, mix properly and further incubate at 37°C for 15 minutes.
5. Pass the cell suspension through 26 G needle to avoid clumping of sample.
6. Acquire the sample on FACSCalibur / Attune NxT.

Preparation of reagents

1. Phosphate buffer saline (0.01 M PBS pH 7.4)

Dissolve in 800 ml distilled water (dH₂O)
Adjust the pH to 7.4 with HCl
Finally, make up the volume to 1 L with dH₂O.
Store at 4°C.

2. 70 % ethanol

Store at 4°C.

3. Propidium iodide (PI): Stock – 400 μg /ml

Dissolve and Aliquots in 1.5 ml Eppendorf tubes
Store at -20°C.
(Precaution: Cover the 1.5 ml Eppendorf tubes with Aluminum foil as PI is light sensitive.

4. RNase: Stock – 1 mg/ml

Dissolve and aliquot in Eppendorf tubes.
Store at -20°C.

Point to remember:

1. The quality of sample should be maintained for better result.
2. Try to make single cell suspension before fixation of the sample to avoid clumping (to prevent the doublets).
3. Handle the tubes with care and wear gloves to avoid contact with skin as PI is mutagenic.

Immunophenotyping

There are three general staining protocols based on type of antigen to be evaluate, nature of Fluorochrome and sample type.

1. Direct Staining:

   This technique involves incubation of cells with fluorochrome conjugated antibody, which recognize and bind to various cell surface antigens. The antibodies are mostly monoclonal antibodies (MAb) and they may be added either single or in multiple combinations depending upon the analysis.

2. Indirect staining:

   In indirect staining technique, the cells are incubated with an unconjugated primary antibody. The primary antibody recognizes and binds to cell surface antigens then the cells are subsequently incubated with a fluorochrome conjugated secondary antibody.

3. Intracellular staining:

   This technique involves detection of intracellular antigens; it done by direct staining method using conjugated antibodies. Cells are initially subjected to a fixation step with paraformaldehyde in order to minimize leakage of proteins out of the cell, following permeabilization by mild detergents such as saponin or Tween 20. The permeabilization allows access of conjugated antibodies to proteins within the cell. The conjugated antibody penetrates and binds to its target within the cell. Intracellular staining technique is very useful in monitoring immune responses and tracking signalling molecules.

Reagents and Instruments

• Cell line or primary cells
• Phosphate buffered saline (PBS, 0.01M pH 7.4)
• FACS Buffer I (for surface staining)
• FACS Buffer II (for intracellular staining)
• 1% Paraformaldehyde
• Refrigerated centrifuge
• Polystyrene round bottom 12 x 75 mm Falcon tubes
• Micropipettes of capacity- 1 ml, 200 μl, 10 μl
• Flow cytometer – FACSCalibur, Attune NxT or FACSAria
• Software - CellQuest Pro (FACSCalibur), Attune NxT (Attune NxT), FACSDiva (FACSAria)

Antibodies

Direct staining:
1. Fluorochrome labelled antibody
2. Isotype matched fluorochrome labelled antibody
Indirect staining:
1. Unlabelled primary antibody
2. Fluorochrome labelled secondary antibody

Procedure

1. Direct Staining

   1. Dispense 1 x 10⁶ Cells in 5ml polystyrene tubes.
   2. Add 1 ml of FACS buffer I and centrifuge the tubes at 1000 rpm for 10 mins at 4°C. Discard the supernatant. (washing)
   3. Resuspend the cells in 100 μl of FACS buffer I.
   4. Add appropriate amount of antibody (the amount of antibody can be decided as per manufacturer’s recommendation and titration)
   5. As negative control, incubate the cells with isotype matched antibody conjugated with same fluorochrome used for the test.
   6. Incubate the cells on ice at 4°C for 45 mins. in dark.
   7. Wash the cells 2 times and finally resuspend in 500 μl of FACS buffer I.
   8. Acquire the sample on flow cytometer as soon as possible.
   9. If the acquisition is not possible on the same day, fix the cells in 500 μl of 1% paraformaldehyde to preserve the cells for several days (this will stabilize the light scatter, prevent quenching and inactivate most biohazardous agents).

2. Indirect Staining

   1. Dispense 1 x 10⁶ Cells in 5ml polystyrene tubes.
   2. Add 1 ml of FACS buffer I and centrifuge the tubes at 1000 rpm for 10 min at 4°C. Discard the supernatant (washing).
   3. Resuspend the cells in 100 μl of FACS buffer I.
   4. Add appropriate amount of unconjugated purified monoclonal antibody specific for antigen to be detected. (the amount of antibody can be change based on manufacturer’s recommendation and titration)
   5. Incubate on ice at 4 ⁰ C for 45 mins.
   6. Wash the cells 2 times with FACS buffer I as described before to remove excess antibody.
   7. Dilute the fluorochrome- labelled secondary antibody as per manufacturer’s instruction in FACS buffer I.
   8. Incubate the cells with diluted secondary antibody (specific for primary antibodies) conjugated with fluorochrome on ice at 4⁰ C for 45 min.
   9. As negative controls, incubate the cells with isotype matched controls conjugated with same fluorochrome used for the test.
   10. Wash the cells 2 times and finally resuspend in 500 μl of FACS buffer I.
   11. Acquire the sample on flow cytometer as soon as possible.
   12. If the acquisition is not possible on the same day, fix the cells in 500 μl of 1% paraformaldehyde to preserve the cells for several days (this will stabilize the light scatter, prevent quenching and inactivate most biohazardous agents).

3. Intracellular staining

   1. Dispense cells in 5ml tubes.
   2. Add 1 ml of FACS buffer I and centrifuge the tubes at 1000 rpm for 10 mins at 4⁰C. Discard the supernatant (washing).
   3. Add 500 μl of 1 % paraformaldehyde to fix the cells.
   4. Incubate on ice at 4⁰C for 15 mins. with intermediate mixing of the tubes.
   5. Wash the cells with FACS buffer I by centrifuging tubes at 1000 rpm for 10 mins. at 4⁰C twice.
   6. After second wash, Add 100 μl FACS buffer II to the cell pellet to permeabilize the cells.
   7. Gently vortex the tubes.
   8. Incubate at room temperature for 5 min.
   9. Add recommended amount of fluorochrome labelled antibody specific for antigen to be detected.
   10. As negative control, incubate the cells with isotype matched controls conjugated with same fluorochrome used for the test.
   11. Incubate on ice at 4°C for 45 mins. in dark.
   12. Wash the cells 2 times and resuspend in 500 μl of FACS buffer I.
   13. Acquire the sample on flow cytometer.

Preparation of reagents:

1. Phosphate buffer saline (0.01 M PBS pH 7.4)

Dissolve in 800 ml distilled water (dH₂O)
Adjust the pH to 7.4 with HCl
Finally, make up the volume to 1 L with dH₂O.
Store at 4°C.

2. FACS buffer I

Store at 4°C.

3. FACS buffer II

Store at 4°C.

4. 1 % paraformaldehyde

Prepare solution in a glass bottle. Heat in microwave oven until the powder dissolves completely. Allow it to cool. Cover the bottle with Aluminium foil to prevent exposure to light. Store at 4°C.
(Precaution: Do not inhale the fumes)

Point to remember:

1. Before starting the experiment, the titration of antibodies should be done according to manufacturer’s instruction for optimum concentration.
2. The recommended controls should be run with every experiment for better analysis e.g. Iso control
3. In case of multicolour analysis use compatible compensation controls.

Annexin V / Propidium iodide staining (For Apoptosis)

Reagents and Instruments:

• Phosphate buffered saline (0.01 M PBS pH 7.4)
• Annexin-V FITC
• Propidium iodide (PI) – stock- 400 μg/ml
• Annexin Binding buffer- 10X
• Refrigerated centrifuge
• Polystyrene round bottom 12 x 75 mm Falcon tubes
• Micropipettes of capacity- 1 ml, 200 μl, 10 μl
• Flow cytometer- FACSCalibur, Attune Nxt
• Softwares – CellQuest Pro, Attune NxT, Flow Jo

Procedure:

1. Wash the cells twice with 1X PBS and then resuspend in 1X Annexin Binding Buffer at a concentration of 1 X 10⁶ cells/ml.
2. Transfer 100 μl of the cell suspension (1 X 10⁵ cells) in another polystyrene tube.
3. Add 2-5 μl of Annexin V FITC.
4. Add 40-50 μg of Propidium Iodide.
5. Gently vortex the cells and incubate for 15 min at room temperature in the dark.
6. Add 300 μl of 1X Annexin Binding Buffer to each tube. Analyze by flow cytometry within 1 hr.

The following controls are used to set up compensation

1. Unstained cells.
2. Cells stained with only Annexin V FITC
3. Cells stained with only Propidium Iodide

Preparation of Reagents:

1. Phosphate buffer saline (0.01 M PBS pH 7.4)

Dissolve the following in 80ml MilliQ Water:

2. Annexin binding buffer 10X

Adjust the pH to 7.4. Make up the volume to 100 ml with MilliQ water. Store at 2-8°C

3. Propidium iodide (PI): Stock – 400 μμg /ml

Dissolve and aliquot in 1.5 ml capacity tubes.
Store at -20°C.
(Precaution: Cover the tube with aluminium foil as PI is photo-sensitive.)

Point to remember:

1. Before starting the experiment, the titration of Annexin V should be done to decide optimal concentration.
2. The pH of Annexin binding buffer should be maintained for better results.
3. Handle with tubes with care and wear gloves to avoid contact with skin as PI is mutagenic.

SOP for Flow Cytometry Facility User

New Flow Cytometry facility users are required to fill up the Project details form before starting the experiment. A hard copy of the same should be submitted with signatures of Principal Investigator/Officer-in-charge/ Scientific officers. The format will be available at the Facility.

Booking for Acquisition and Analysis of samples

1. Booking has to be done in Booking register that is available in the Facility.
2. Booking can be done one-week prior to the date of sample acquisition.
3. Booking slots are available for both acquisition and sorting. In a given day, only one booking slot of 2 hrs is allowed. Additional time will be entertained based on the nature of the experiments and number samples depending on the availability of the slots.
4. Cancellation of booking if any should be informed at least one-day prior of date of acquisition.
5. If any queries, please contact Facility staff.

Sample Preparation for Flow Cytometry

1. Try to prepare single cells suspension and avoid clumping. Users are Recommended to pass the cells through filters or strainers or insulin syringe.
2. Sample concentration should be maintained for better results. (e.g. 1 X10⁶ Cells / 300-500 μl for acquisition depending upon the cell type)
3. For sorting carry sufficient number of collection tubes with appropriate quantity of media or buffer.
4. User can use 15 ml tubes, 5 ml FALCON tubes, Eppendorf 1.5 /2 ml, and 96 well plates for collection of cells.

General Guidelines

• Suitable Controls should be carry at the time of acquisition and if any queries please contact Facility Staff.
• Data can be collected in FCS format in the CD immediately after the sample acquisition.
• Users can use softwares Flowjo, FACS DIVA, Attune NxT, Modfit LT 5.0, Cells Quest Pro, for analysis with prior booking.

Dr. Sanjeev K Waghmare
Principal Investigator
Scientific Officer’ F’
Officer In-Charge
Email: swaghmare[at]actrec[dot]gov[dot]in
Phone:022-27405122

Flow Cytometry Facility, ACTREC
Email: flowcytometry[at]actrec[dot]gov[dot]in
Phone: 022-27405000 Ext. 5316

TRANSMISSION ELECTRON MICROSCOPE Make: JEOL Model: JEM 1400 PLUS

JEM 1400 PLUS is a Transmission electron microscope (JEOL, Japan) functioning at 120kV with magnification up to 12,00,000 X and 0.2nm resolution.

Specifications

Applications

• Imaging of resin embedded cells and tissues.
• Imaging of negatively stained small particles (<100nm) like bacteriophages, exosomes, nanoparticles, proteins and DNA.
• Acquisition of immunogold labelled macromolecules to detect specific localization.
• Elemental detection by Bruker EDS system.

EQUIPMENT: ULTRAMICROTOME Make: Leica Model: UC7

Ultramicrotome provides high-quality preparation of ultra-thin or semi-thin sections for transmission electron microscopy. The desired thickness of the section can be selected with touchscreen control unit and sections can be cut using glass or diamond knives with motorized knife stage. Majorly, 70nm ultra-thin sections are cut for electron microscopy.

Application

• Ultra-thin and semi-thin sectioning of resin embedded cells and tissues.

Additional equipment’s

	Leica EM TRIM2 auto-trimmer – for trimming the resin blocks,
	Leica EM KMR3 – Glass knife maker – for making glass knives.
	Leica EM MP Hotplate – for heating the resin sections.

GLOW DISCHARGE UNIT Make: Ted Pella Model: PELCO easiGlow

The PELCO easiGlow uses an automated and quick cycle with fully selectable parameters for one of the most common glow discharge applications, making TEM support films or grids hydrophilic using air.

Applications

• To make TEM support films or grids hydrophilic using air
• Cleaning TEM grids
• Adhesion and orientation of proteins, nucleic acids and extra cellular vesicles.
• TEM grid preparation for nanoparticle studies

• The facility carries out sample preparation for routine electron microscopy, including resin block making (solid tissues, monolayer cell cultures, and cell suspensions) followed by ultrathin sectioning using Leica UC-7 ultramicrotome, contrasting, and acquisition.
• The facility also carries out negative staining for small particles (<100nm) like bacteriophages, exosomes, nanoparticles, proteins and DNA.
• The facility provides acquisition of immunogold labelled macromolecules to detect specific localization.
• The facility provides elemental detection by Bruker EDS system

The major areas of electron microscopy analysis are,

• Ultrastructure study of cell and tissue,
• Studying the details at organelle level like mitochondrial changes, ER - mitochondrial interactions, mitochondrial cristae, cell membrane, nuclear architecture, golgi complexes etc.,
• To check events of autophagy, apoptosis or necroptosis,
• Characterization of exosomes, bacteriophages and nanoparticles,
• Uptake and effect of nanoparticles / drugs / radiation on cells or tissue,
• EDS analysis of nanoparticles

Electron Microscopy ATLAS

External users: Please write to the facility regarding your requirement for electron microscopy. The facility will provide the details as per the analysis requirement. The users are requested to discuss over email regarding their study and sample submission followed by booking a slot accordingly. The samples need to be submitted along with a duly filled requisition form. For quotation, a duly filled requisition form can be mailed to the facility email address (tem.facility@actrec.gov.in). For requisition form, please click the link below,

Internal users (ACTREC):

• For routine EM, please check with the facility regarding the sample submission requirements as per the study.
• For negative staining, please book a slot after discussing with the facility for sample submission. Only three samples will be accepted per slot.

Samples should be accompanied by a duly filled requisition form and online requisition.

Requisition form

18% GST to be included in addition to the following charges.

Mode of Payment

External users: The users should pay the charges against the quotation or invoice to the following account within a week of the invoice. The users should share the transaction details with the facility once the payment is done. The acquisition will be carried out after the receipt of the payment is confirmed. For e-payment details please click the link below.

Epayment details

Facility-in-charge:
Siddhi A. Redkar
Technical Officer ‘D’
Electron Microscope Facility
(Khanolkar Shodhika - 68)
ACTREC, TMC
Plot No.1 & 2, Sector 22
Kharghar, Navi Mumbai - 410 210.
022-27405000 / 022-68735000
Extension : 5545
Email : tem.facility@actrec.gov.in

EM Staff:
Ms. Arpana Kadam, Scientific Assistant ‘C’
Mr. Shridhar Nadkar, Technician ‘F’

2026

• Gupta RJ, Nair H, Sarhadi TR, Nagotu S, Gupta PP, Mishra RM, Dantu SC, Kale A. R5 and R7 positions on fluoroquinolone scaffolds drive F-actin filament disruption. Sci Rep. 2026 Feb 18;16(1):9517.PMID: 41708669; PMCID: PMC13004907.
• Dutta D, Maity A, Gupta SK, Gera P, Jain H, Bagal B, Chatterjee G, Rajpal S, Nayak L, Punatar S, Bandyopadhyay S, Chakraborty A, Chendamarai E, Balasundaram N, Gota V, Khattry N, Sengar M, Mathews V, Hasan SK. Venetoclax induces mitochondrial apoptosis and autophagy to overcome arsenic trioxide resistance in acute promyelocytic leukemia. J Transl Med. 2026 Jan 12;24(1):56.PMID: 41527127; PMCID: PMC12797632.



2025

• Meher RK, Maharana J, Kovvuri J, Kumar KN, Pradhan T, Kailaje V, Ingle A, Sen S, Barkume M, Patkar M, Vaibhaw A, Mir SA, Ghosh A, Kamte S, Kamal A, Kode J. A Phenstatin-based indole-chalcone hybrid as novel anti-inflammatory agent targeting the NLRP3 Inflammasome: Leads from computational and preclinical studies. Bioorg Chem. 2026 Mar;170:109429.Epub 2025 Dec 26. PMID: 41500010.
• Nagare M, Sahoo M, Sengar M, Punatar S, Khattry N, Gokarn A, Bagal B, Jain H, Mirgh S, Epari S, Shet T, Pradhan T, Shirsat S, Barkume M, Mathen C, Gera P, Verma RK, Saldanha E, Chandrani P, Gawde J, Chiplunkar S, Kode J. Defining alteration in bone marrow mesenchymal stem cells (MSC) from acute myeloid leukemia and exploring cultured MSC-conditioned media as a novel anti-leukemia therapy agent. Cancer Immunol Immunother. 2026 Feb 23;75(3):83.PMID: 41729306; PMCID: PMC12929743
• George A, Nair S, Prabhash K, Thakur S, Gera P, Singh A, Chaturvedi P, Rane S, Pradhan T, Sen S, Barkume M, Shetty D, Chaubal K, Ghosh A, Kamte S, Kode J (2026). Generation and characterization of early stage oral cancer cell line of buccal mucosa of Indian origin. Human Cell. 39: 21. PMID: 41410801
• Panigrahi GC, Joshi A, Malhotra D, Gota V (2025). Mitocurcumin induces ROS-/JNK-mediated paraptosis to overcome chemoresistance in non-small cell lung cancer. Naunyn-Schmiedeberg's Archives of Pharmacology. PMID: 41422457.
• Shenoy PS, Dash S, Joshi D, Rekhi B, Gota V, Ray P (2025). Withaferin A exerts cytotoxicity in single/multidrug-resistant gastric and ovarian cancer cells and tumor xenografts through the AKT-NF-κB-STAT3-survivin axis. Molecular Carcinogenesis. 64(12):2037-2051.PMID: 41025926.
• Singh V, Ansari MM, Dutta D, Rajmani RS, Singh A, Singh BN. M. bovis FadR mutant exhibits an altered colony morphotype and increased virulence. Tuberculosis (Edinb). 2025 Dec;155:102689. Epub 2025 Sep 6. PMID: 40939556.
• Chaudhary N, Mandal D, Choudhary B, Patra S, Jain D, Poonia P, Shaikh S, Tekalkar S, Malvankar S, Shivashankar A, Jog E, Pilankar L, Thorat R, Kailje V, Khanna S, Manna S, Khan B, Jadhav A, Sharma K, Ramu S, Sahoo S, Jolly MK, Dalal SN, Sinha S, Nair N, Ruppin E, Lamballe F, Maina F, Verma N (2025). Low-GPX4 drives a sustained drug-tolerant persister state in TNBC by a targetable adaptive FSP1 upregulation. Redox Biology 87: 103864. PMID: 40972157.
• Chaudhary N, Mandal D, Choudhary B, Patra S, Jain D, Poonia P, Shaikh S, Tekalkar S, Malvankar S, Shivashankar A, Jog E, Pilankar L, Thorat R, Kailje V, Khanna S, Manna S, Khan B, Jadhav A, Sharma K, Ramu S, Sahoo S, Jolly MK, Dalal SN, Sinha S, Nair N, Ruppin E, Lamballe F, Maina F, Verma N (2025). Low-GPX4 drives a sustained drug-tolerant persister state in TNBC by a targetable adaptive FSP1 upregulation. Redox Biology 87: 103864. PMID: 40972157.
• Patnaik C, Reddy BPK, Gupta A, Rath S, Neekhra S, Chakrabarty S, Chaudhari P, Asad S, Ingle A, Srivastava R, De A. Non-invasive imaging-guided preclinical assessments reveal high performance photothermal effect of an Au-solid lipid nanomaterial. Nanoscale. 17(46):26658-26677. PMID: 40792496.
• Jaiswal MA, Karn A, Das A, Kumari A, Tiwari S, Dalal SN (2025). 14-3-3ɛ inhibits premature centriole disengagement by inhibiting the activity of Plk1 and Separase. Journal of Cell Science. 138(14):jcs263808. PMID: 40576425.
• Mehta D, Paradkar A, Nayak P, Rekhi B, Mohanty B, Chaudhari P, Waghmare SK. Establishment and molecular characterization of the novel cutaneous squamous cell carcinoma cell line from advanced-stage Indian patient. Human Cell.38 (4):108. PMID: 40413685.
• Joshi P, Bane S, Chaturvedi P, Gera P, Waghmare SK (2025). Establishment and characterization of patient-derived tongue squamous cell carcinoma cell lines. Human Cell. 38(4):102. PMID: 40394426.
• Dahara R, Bhattacharyya D (2025). Intra-Golgi Golgin PpSgm1 and GRIP domain Golgin PpImh1 synergistically mediate Golgi cisternal stacking. Journal of Cell Science.138 (11):jcs263612. PMID: 40270185.
• Anaganti N, Ujaoney AK, Padwal MK, Basu B. Biochemical characterization and functional insights into DNA substrate-specific activities of a unique radiation-inducible DR1143 protein from Deinococcus radiodurans. Int J Biol Macromol. 2025 May;310(Pt 1):143214.Epub 2025 Apr 16. PMID: 40250669.
• Jog E, Jainarayanan AK, Ferlita A, Chakraborty A, Dalwai A, Yahya S, Shivashankar A, Choudhary B, Chandramouli A, Kazi M, Jain D, Khapre N, Akshaya B, Khan B, Gera P, Patil P, Thorat R, Verma N, Sehgal L, Saklani A, Kamat S, Dalal SN, Choudhary N (2025). Inhibiting de novo lipogenesis identifies a therapeutic vulnerability in therapy-resistant colorectal cancer. Redox Biology.79:103458.PMID: 39705849
• Chaudhary N, La Ferlita A, Choudhary BS, Jog E, Kazi M, Yahya S, Dalwai A, Ostwal V, Kumar S, Redkar S, Khapare N, Kailaje V, B A, Gera P, Bal M, Verma N, Thorat R, Saklani A, Sehgal L, Dalal SN (2025). Patient-derived Organoids and Xenografts Uncover Therapeutic Vulnerabilities in Colorectal Signet Ring Cell Carcinomas. Clinical Cancer Research. 31(7):1359-137 PMID: 39879477.



2024

• Arora S, Nagarkar P, D'Souza JS. Recombinant human FOXJ1 protein binds DNA, forms higher-order oligomers, has gel-shifting domains and contains intrinsically disordered regions. Protein Expr Purif. 2025 Mar;227:106622. Epub 2024 Nov 15. PMID: 39549898.
• Shetake NG, Das SK, Kumar A, Pandey BN. Nano-inducer of ferroptosis for targeted chemotherapy of human triple negative breast carcinoma. Biomater Adv. 2024 Jul;161:213868. Epub 2024 Apr 20. PMID: 38677038.
• Meher K, Paithankar H, Hosur RV, Lopus M. Antiproliferative efficacy and mechanism of action of garlic phytochemicals-functionalized gold nanoparticles in triple-negative breast cancer cells. Biomed Mater. 2024 Apr 29;19(3). doi: 10.1088/1748-605X/ad3ff9. PMID: 38682577.
• Radha G, Pragyandipta P, Naik PK, Lopus M. The mode of action of sorafenib in MDA-MB-231 breast carcinoma cells involves components of apoptotic, necroptotic and autophagy-dependent cell death pathways. Exp Cell Res. 2024 Nov 1;443(1):114313. Epub 2024 Oct 30. PMID: 39486634.
• Meher K, Radha G, Lopus M. Induction of autophagy-dependent and caspase- and microtubule-acetylation-independent cell death by phytochemical-stabilized gold nanopolygons in colorectal adenocarcinoma cells. Nanoscale. 2024 Apr 25;16(16):7976-7987. PMID: 38567463.
• Radha G, Pragyandipta P, Naik PK, Lopus M. Biochemical and in silico analysis of the binding mode of erastin with tubulin. J Biomol Struct Dyn. 2025 Aug;43(13):6522-6529. Epub 2024 Feb 20. PMID: 38375607.
• Lonare A, Raychaudhuri K, Shah S, Madhu G, Sachdeva A, Basu S, Thorat R, Gupta S, Dalal SN. 14-3-3σ restricts YY1 to the cytoplasm, promoting therapy resistance, and tumor progression in colorectal cancer. Int J Cancer. 2025 Feb 1;156(3):623-637. doi: 10.1002/ijc.35176. Epub 2024 Sep 6. PMID: 39239852; PMCID: PMC11622004.
• Phadte P, Bishnu A, Dey P, M M, Mehrotra M, Singh P, Chakrabarty S, Majumdar R, Rekhi B, Patra M, De A, Ray P. Autophagy-mediated ID1 turnover dictates chemo-resistant fate in ovarian cancer stem cells. J Exp Clin Cancer Res. 2024 Aug 10;43(1):222.PMID: 39123206; PMCID: PMC11316295.
• Ketkar M, Desai S, Rana P, Thorat R, Epari S, Dutt A, Dutt S. Inhibition of PERK-mediated unfolded protein response acts as a switch for reversal of residual senescence and as senolytic therapy in glioblastoma. Neuro Oncol. 2024 Nov 4;26(11):2027-2043.PMID: 39021199; PMCID: PMC11534322.
• Nagar V, Godambe LP, Newase SK, Tyagi A. Characterization and Genome Analyses of the Novel Phages P2 and vB_AhydM-H1 Targeting Aeromonas hydrophila. Phage (New Rochelle). 2024 Sep 16;5(3):162-172.PMID: 39372357; PMCID: PMC11447392.
• Garg M, Gandhi K, Gera P, Jadhav SM, Mohanty B, Gurjar M, Sandupatla B, Gala R, Chaudhari P, Prasad M, Chinnaswamy G, Gota V. Implications of chronic moderate protein-deficiency malnutrition on doxorubicin pharmacokinetics and cardiotoxicity in early post-weaning stage. Life Sci. 2024 Aug 1;350:122765. Epub 2024 Jun 1. PMID: 38830506.



2023

• Rauniyar S, Pansare K, Sharda A, Singh SR, Saha P, Chilakapati MK, Gupta S. Raman Spectroscopy Revealed Cell Passage-Dependent Distinct Biochemical Alterations in Radiation-Resistant Breast Cancer Cells. ACS Omega. 2023 Feb 3;8(6):5522-5532. PMID: 36816694; PMCID: PMC9933476.
• Dubey S, Mishra N, Goswami N, Siddiqui MQ, Varma AK. Multimodal approach to characterize the tetrameric form of human PML-RBCC domain and ATO-mediated conformational changes. Int J Biol Macromol. 2022 Dec 31;223(Pt A):468-478.Epub 2022 Nov 8. PMID: 36356867.
• Mishra N, Dubey S, Kumari A, Khan MA, Kuligina ES, Preobrazhenskaya EV, Romanko AA, Yadav LR, Sarin R, Imyanitov EN, Varma AK. Structural implications of amyloidogenic rare variants Ser282Leu and Gln356Arg identified in h-BRCA1. Proteins. 2024 Apr;92(4):540-553. Epub 2023 Dec 1. PMID: 38037760.
• Navarange SS, Bane SM, Mehta D, Shah S, Gupta S, Waghmare SK. Epithelial-to-mesenchymal transition status correlated with ultrastructural features, and TP53 mutation in patient-derived oral cancer cell lines. Mol Biol Rep. 2023 Oct;50(10):8469-8481.Epub 2023 Aug 28. PMID: 37639153.
• Ujaoney AK, Anaganti N, Padwal MK, Basu B. Deinococcus lineage and Rad52 family-related protein DR0041 is involved in DNA protection and compaction. Int J Biol Macromol. 2023 Sep 1;248:125885. Epub 2023 Jul 18. PMID: 37473881.
• Chatterjee S, Ganguly A, Bhattacharyya D. Reprogramming nucleolar size by genetic perturbation of the extranuclear Rab GTPases Ypt6 and Ypt32. FEBS Lett. 2024 Feb;598(3):283-301. Epub 2023 Dec 1. PMID: 37994551.



2022

• Dongre HN, Mahadik S, Ahire C, Rane P, Sharma S, Lukmani F, Patil A, Chaukar D, Gupta S, Sawant SS. Diagnostic and prognostic role of protein and ultrastructural alterations at cell-extracellular matrix junctions in neoplastic progression of human oral malignancy. Ultrastruct Pathol. 2022 Sep 3;46(5):476-489. Epub 2022 Sep 1. PMID: 36049041.
• Shukla P, Dange P, Mohanty BS, Gadewal N, Chaudhari P, Sarin R. ARID2 suppression promotes tumor progression and upregulates cytokeratin 8, 18 and β-4 integrin expression in TP53-mutated tobacco-related oral cancer and has prognostic implications. Cancer Gene Ther. 2022 Dec;29(12):1908-1917. Epub 2022 Jul 22. PMID: 35869277.
• Chaudhary N, Joshi N, Doloi R, Shivashankar A, Thorat R, Dalal SN. Plakophilin3 loss leads to an increase in autophagy and radio-resistance. Biochem Biophys Res Commun. 2022 Sep 10;620:1-7. Epub 2022 Jun 22. PMID: 35772211.



2021

• Ud Din Farooqee SB, Christie J, Venkatraman P. PSMD9 ribosomal protein network maintains nucleolar architecture and WT p53 levels. Biochem Biophys Res Commun. 2021 Jul 23;563:105-112. Epub 2021 May 30. PMID: 34077860.
• Sulkshane P, Pawar SN, Waghole R, Pawar SS, Rajput P, Uthale A, Oak S, Kalkar P, Wani H, Patil R, Nair S, Rane P, Teni T. Elevated USP9X drives early-to-late-stage oral tumorigenesis via stabilisation of anti-apoptotic MCL-1 protein and impacts outcome in oral cancers. Br J Cancer. 2021 Aug;125(4):547-560. Epub 2021 Jun 2. PMID: 34079080; PMCID: PMC8367974.
• Rajendra J, Ghorai A, Dutt S. 14-3-3ζ negatively regulates mitochondrial biogenesis in GBM residual cells. Heliyon. 2021 Nov 12;7(11):e08371. PMID: 34825085; PMCID: PMC8605068.
• Bishnu A, Phadte P, Dhadve A, Sakpal A, Rekhi B, Ray P. Molecular imaging of the kinetics of hyperactivated ERK1/2-mediated autophagy during acquirement of chemoresistance. Cell Death Dis. 2021 Feb 8;12(2):161. PMID: 33558461; PMCID: PMC7870816.
• Bose K, Wagh A, Mishra V, Dutta S, Parui AL, Puja R, Mudrale SP, Kulkarni SS, Gai PB, Sarin R. Loss of GSK-3β mediated phosphorylation in HtrA2 contributes to uncontrolled cell death with Parkinsonian phenotype. Int J Biol Macromol. 2021 Jun 1;180:97-111. Epub 2021 Mar 12. PMID: 33716130.



2020

• Khare D, Kumar R, Acharya C. Genomic and functional insights into the adaptation and survival of Chryseobacterium sp. strain PMSZPI in uranium enriched environment. Ecotoxicol Environ Saf. 2020 Mar 15;191:110217. Epub 2020 Jan 27. PMID: 32001422.
• Salunkhe S, Mishra SV, Ghorai A, Hole A, Chandrani P, Dutt A, Chilakapati M, Dutt S. Metabolic rewiring in drug resistant cells exhibit higher OXPHOS and fatty acids as preferred major source to cellular energetics. Biochim Biophys Acta Bioenerg. 2020 Dec 1;1861(12):148300. Epub 2020 Aug 25. PMID: 32858000.
• Kaur E, Nair J, Ghorai A, Mishra SV, Achareker A, Ketkar M, Sarkar D, Salunkhe S, Rajendra J, Gardi N, Desai S, Iyer P, Thorat R, Dutt A, Moiyadi A, Dutt S. Inhibition of SETMAR-H3K36me2-NHEJ repair axis in residual disease cells prevents glioblastoma recurrence. Neuro Oncol. 2020 Dec 18;22(12):1785-1796. PMID: 32458986; PMCID: PMC7746947.
• Sharda A, Rashid M, Shah SG, Sharma AK, Singh SR, Gera P, Chilkapati MK, Gupta S. Elevated HDAC activity and altered histone phospho-acetylation confer acquired radio-resistant phenotype to breast cancer cells. Clin Epigenetics. 2020 Jan 3;12(1):4.PMID: 31900196; PMCID: PMC6942324.
• Roy Chowdhury S, Bhattacharjee C, Casler JC, Jain BK, Glick BS, Bhattacharyya D. ER arrival sites associate with ER exit sites to create bidirectional transport portals. J Cell Biol. 2020 Apr 6;219(4):e201902114. PMID: 32328626; PMCID: PMC7147096.



2019

• Pansare K, Gardi N, Kamat S, Dange P, Previn R, Gera P, Kowtal P, Amin K, Sarin R. Establishment and genomic characterization of gingivobuccal carcinoma cell lines with smokeless tobacco associated genetic alterations and oncogenic PIK3CA mutation. Sci Rep. 2019 Jun 4;9(1):8272. PMID: 31164688; PMCID: PMC6547758.
• Jain BK, Dahara R, Bhattacharyya D. The golgin PpImh1 mediates reversible cisternal stacking in the Golgi of the budding yeast Pichia pastoris. J Cell Sci. 2019 Sep 6;132(17):jcs230672. PMID: 31391238.



2018

• Kirolikar S, Prasannan P, Raghuram GV, Pancholi N, Saha T, Tidke P, Chaudhari P, Shaikh A, Rane B, Pandey R, Wani H, Khare NK, Siddiqui S, D'souza J, Prasad R, Shinde S, Parab S, Nair NK, Pal K, Mittra I. Prevention of radiation-induced bystander effects by agents that inactivate cell-free chromatin released from irradiated dying cells. Cell Death Dis. 2018 Nov 15;9(12):1142. PMID: 30442925; PMCID: PMC6238009.
• Ali M, Sadhu B, Boda A, Tiwari N, Das A, Musharaf Ali SK, Bhattacharya D, Pandey BN, Kumar A. Thorium decorporation efficacy of rationally-selected biocompatible compounds with relevance to human application. J Hazard Mater. 2019 Mar 5;365:952-961. Epub 2018 Nov 13. PMID: 30616306
• Jain BK, Thapa PS, Varma A, Bhattacharyya D. Identification and characterization of GRIP domain Golgin PpImh1 from Pichia pastoris. Yeast. 2018 Aug;35(8):499-506.Epub 2018 May 21. PMID: 29707810.
• Chandwadkar P, Misra HS, Acharya C. Uranium biomineralization induced by a metal tolerant Serratia strain under acid, alkaline and irradiated conditions. Metallomics. 2018 Aug 15;10(8):1078-1088. PMID: 29999065.
• Kolhe N, Zinjarde S, Acharya C. Responses exhibited by various microbial groups relevant to uranium exposure. Biotechnol Adv. 2018 Nov 15;36(7):1828-1846.Epub 2018 Jul 12. PMID: 30017503.
• Sawant S, Dongre H, Ahire C, Sharma S, Jamghare S, Kansara Y, Rane P, Kanojia D, Patil A, Chaukar D, Gupta S, D'Cruz A, Vaidya M, Dongre P. Alterations in desmosomal adhesion at protein and ultrastructure levels during the sequential progressive grades of human oral tumorigenesis. Eur J Oral Sci. 2018 Aug;126(4):251-262. Epub 2018 Jun 15. PMID: 29905981.
• Salunkhe S, Mishra SV, Nair J, Ghosh S, Choudhary N, Kaur E, Shah S, Patkar K, Anand D, Khattry N, Hasan SK, Dutt S. Inhibition of novel GCN5-ATM axis restricts the onset of acquired drug resistance in leukemia. Int J Cancer. 2018 May 15;142(10):2175-2185. Epub 2018 Jan 13. PMID: 29297932.



2017

• Vishal SS, Tilwani S, Dalal SN. Plakoglobin localization to the cell border restores desmosome function in cells lacking 14-3-3γ. Biochem Biophys Res Commun. 2018 Jan 8;495(2):1998-2003. Epub 2017 Dec 15. PMID: 29253567.
• Shanavas A, Rengan AK, Chauhan D, George L, Vats M, Kaur N, Yadav P, Mathur P, Chakraborty S, Tejaswini A, De A, Srivastava R. Glycol chitosan assisted in situ reduction of gold on polymeric template for anti-cancer theranostics. Int J Biol Macromol. 2018 Apr 15;110:392-398. Epub 2017 Nov 22. PMID: 29174361.
• Chauhan DS, De A et al., 2017. NIR light triggered shrinkable thermoresponsive PNVCL nanoshells for cancer theranostics. RSC Advances, 7(70), 44026–34.
• Gurjar M, Raychaudhuri K, Mahadik S, Reddy D, Atak A, Shetty T, Rao K, Karkhanis MS, Gosavi P, Sehgal L, Gupta S, Dalal SN. Plakophilin3 increases desmosome assembly, size and stability by increasing expression of desmocollin2. Biochem Biophys Res Commun. 2018 Jan 1;495(1):768-774. Epub 2017 Nov 14. PMID: 29146182.
• Sawant SS, Dongre H, Ahire C, Sharma S, Kannan S, Mahadik S, Chaukar D, Lukmani F, Patil A, D'Cruz A, Vaidya MM, Dongre P. A nomogram for predicting the risk of neck node metastasis in pathologically node-negative oral cavity carcinoma. Oral Dis. 2017 Nov;23(8):1087-1098. Epub 2017 Jul 5. PMID: 28580710.



2016

• Mukhopadhyay A, Sehgal L, Bose A, Gulvady A, Senapati P, Thorat R, Basu S, Bhatt K, Hosing AS, Balyan R, Borde L, Kundu TK, Dalal SN. 14-3-3γ Prevents Centrosome Amplification and Neoplastic Progression. Sci Rep. 2016 Jun 2;6:26580. PMID: 27253419; PMCID: PMC4890593.
• Sawant S, Dongre H, Singh AK, Joshi S, Costea DE, Mahadik S, Ahire C, Makani V, Dange P, Sharma S, Chaukar D, Vaidya M. Establishment of 3D Co-Culture Models from Different Stages of Human Tongue Tumorigenesis: Utility in Understanding Neoplastic Progression. PLoS One. 2016 Aug 8;11(8):e0160615. PMID: 27501241; PMCID: PMC4976883.
• Sulkshane P, Teni T. BH3 mimetic Obatoclax (GX15-070) mediates mitochondrial stress predominantly via MCL-1 inhibition and induces autophagy-dependent necroptosis in human oral cancer cells. Oncotarget. 2016 Aug 5;8(36):60060-60079.PMID: 28947954; PMCID: PMC5601122.
• Dmello C, Sawant S, Alam H, Gangadaran P, Tiwari R, Dongre H, Rana N, Barve S, Costea DE, Chaukar D, Kane S, Pant H, Vaidya M. Vimentin-mediated regulation of cell motility through modulation of beta4 integrin protein levels in oral tumor derived cells. Int J Biochem Cell Biol. 2016 Jan;70:161-72. Epub 2015 Nov 29. PMID: 26646105.



2015

• Rengan AK, Bukhari AB, Pradhan A, Malhotra R, Banerjee R, Srivastava R, De A. In vivo analysis of biodegradable liposome gold nanoparticles as efficient agents for photothermal therapy of cancer. Nano Lett. 2015 Feb 11;15(2):842-8. Epub 2015 Jan 7. PMID: 25554860.

Facility Team:

• Mrs. Vaishali Kailaje, Scientific Assistant,’F’ , Facility In charge
• Mrs. Tanuja Durve, Scientific Assistant, ‘E’
• Ms. Rajashree Sawant, Scientific Assistant, ‘B’

Facility Board Members:

• Dr. Sorab Dalal, Scientific Officer, ‘G’, Deputy Director, CRI
• Dr. Rohan Khadilkar, Scientific Officer, ‘E’

Facility Reporting Officer:

• Mr. Uday Dandekar. Engineer, ‘H’

Student’s representatives:

• Ms. Shivali Mishra
• Mr. Sourav Chakraborty

ZEISS LSM 980 AS2 Confocal

The ZEISS LSM 980 with Airyscan 2 is a next-generation laser scanning confocal microscope designed for high-sensitivity, and super-resolution fluorescence imaging. Featuring the innovative Airyscan 2 detector, the system captures significantly more light and spatial information than conventional confocal microscopes, delivering enhanced resolution, superior signal-to-noise ratio, and improved imaging speed with minimal phototoxicity. Integrated with automated stage control, Definite Focus technology, spectral detection, and live-cell imaging capabilities, the LSM 980 is an ideal platform for advanced biological research.

     

CONFOCAL SYSTEMS Nikon AX Confocal

The Nikon AX Confocal Microscope is a state-of-the-art laser scanning confocal imaging system engineered for high-speed, high-resolution, and large field-of-view imaging. Built on the Nikon Ti2-Eclipse fully motorized inverted microscope, with 25 mm ultra-large field-of-view galvo scanner, up to 8192 × 8192-pixel image acquisition, imaging speeds up to 10 fps, 360° scan field rotation, Perfect Focus System (PFS) for long-term live-cell imaging, environmental incubator for 35 mm dishes and multiwell plates.

     

LSM780 Confocal

 

• High-resolution fluorescence imaging of cells and tissues
• Optical sectioning and three-dimensional (3D) reconstruction of cells, tissues, organoids spheroids etc.
• Live-cell and short and long-term time-lapse imaging to study migration, division, autophagy and dynamics of organelles and proteins.
• Multi-color fluorescence imaging
• Protein localization and intracellular trafficking studies
• Colocalization and interaction analysis of biomolecules (FRET)
• Fluorescence Recovery After Photobleaching (FRAP) to study mobility and diffusion of proteins.
• Cell morphology and cytoskeletal organization studies
• Organelle imaging (mitochondria, nucleus, lysosomes, Golgi, ER, etc.)
• Cancer biology and tumor microenvironment research
• Drug screening and pharmacological studies
• Large-area tissue imaging using tile scanning
• Quantitative fluorescence intensity measurements
• Biomaterial and scaffold imaging
• Reflection imaging of non-fluorescent specimens

WIDEFIELD SYSTEMS - Axio Imager Z.1 Upright Microscope, Carl Zeiss

The AxioImager Z.1 is an upright fluorescence microscope used for imaging of cells, tissues, and diverse biological specimens. Partially motorized components, equipped with ZEISS optics - 6 position emission filter wheel and objective turret.

     

BX63, Upright Microscope, Olympus

The Olympus BX63 is a partially motorized upright microscope used for imaging of biological specimens - cells, tissues, organism etc. with 8 position emission filter wheel, LED as illumination sources for fluorescence light.

     

IX73 Inverted Microscope, Olympus

The Olympus IX73 is a compact inverted fluorescence microscope designed for routine cell culture observation and basic fluorescence imaging. Equipped with phase contrast, fluorescence illumination, and a color digital camera

   

Axio Observer Inverted Microscope, Carl Zeiss

The Carl Zeiss Axio Observer is a fully motorized inverted fluorescence microscope used for advanced live-cell and fluorescence imaging. Featuring an environmental chamber, Colibri LED illumination and Definite focus for z-drift compensation.

     

Nikon Eclipse Ti Inverted Microscope

Nikon Eclipse Ti Inverted Microscope is a Live-cell and fluorescence imaging system. Equipped with an environmental chamber that maintains physiological conditions (37°C and 5% CO₂), the system enables long-term imaging of living cells. It has the Perfect Focus for Z drift compensation.

 

Incucyte

This is a widefield inverted live cell system engineered with long term high throughput imaging in mind. Housed inside a tissue culture incubator, the imaging module maintains the most stable imaging conditions possible (37ºC temperature, humidity and 5% CO2). The system comes with a selection of inserts allowing you to image your cells in a range of vessels (ranging from 35mm dishes to 384 well plates). It automatically captures and analyses images of living cells in real time for days, weeks, or months. It has a optical design wherein the cells remain stationary while optics move. Multiple imaging modes – HD phase plus red and green fluorescence. It can accommodate multiple users at a time with different experiment set ups and configurations

           

Dedicated analysis workstations equipped with Imaris, Arivis Pro

Imaris for advanced 3D visualization, segmentation, cell tracking, and quantitative image analysis.

Arivis Pro for AI-assisted segmentation, analysis of large image datasets, multidimensional visualization, and automated workflows.

Advanced Microscopy

Confocal laser scanning microscopy and improved-resolution imaging of subcellular structures with Airy Scan hardware. Multi-channel fluorescence imaging, Spectral imaging and spectral unmixing

Widefield fluorescence microscopy - Brightfield, phase-contrast, and DIC imaging, Tile scan - large-area imaging.
Time-lapse imaging - long-term live-cell imaging under controlled temperature and CO₂ conditions with automated multilocation and multichannel imaging.

Image Processing

Image deconvolution, noise reduction and AI-assisted denoising, spectral unmixing,3D reconstruction and rendering, image stitching, movie generation and image annotation

Quantitative Image Analysis

2D, 3D, and 4D image visualization, cell segmentation and object detection, colocalization analysis, fluorescence intensity measurements, surface, volume, and morphology quantification, AI-assisted image segmentation and analysis.

Functional Imaging

• Fluorescence Recovery After Photobleaching (FRAP) / FRET (Förster Resonance Energy Transfer)
• Cell migration and wound-healing assays
• Chemotaxis and proliferation studies
• Confluence and viability analysis using Incucyte

User Training and Technical Support

• Instrument operation training
• Experimental planning and consultation
• Assistance in optimizing imaging protocols for diverse biological samples
• Selection of appropriate imaging techniques
• Assistance with image acquisition and optimization
• Image analysis guidance
• Troubleshooting and technical support

Data Management

We are happy to assist you with your microscopy and image analysis requirements. Feel free to contact us by email or phone for any queries related to the facility or our services.

In-house Certified Users

Certified in-house users can access the facility independently after regular working hours, as well as on weekends and public holidays, subject to facility guidelines and instrument availability.

External Users

Researchers from external institutes are welcome to use the facility. Please email us with a brief description of your project, your imaging requirements, and the microscope(s) you wish to use. Our team will guide you in selecting the most suitable system and assist with scheduling your experiments.

2026

• Mehta D, Paradkar A, Rekhi B, Nayak P, Mohanty B, Chaudhari P, Gera P, Rane S, Chaturvedi P, Waghmare SK. Histone methylation defines c-Jun/Sox2/Hif1α axis that controls stemness and tumor progression in squamous cell carcinoma. Nature Communications. PMID: 42045185
• Dey P, Bishnu A, Patel J, Mishra S, Gadewal N, Ray P, Gupta S, De A. Hotspot mutations in HER2 interfaces destabilize structure, causing breast cancer treatment failure. Oncogene. 45(3):414-430. PMID: 41392272
• Mehrotra M, Shenoy P, Sakpal A, Chakraborty S, Ray P. p53-R248W evades doxorubicin mediated cell death through collective alteration in TOP2A and CYP1A1 genes in cancer cells. Biochemical and Biophysical Research Communications. 819: 153759. PMID: 42019355
• Manasi Nagare, Monalisa Sahoo, Manju Sengar, Sachin Punatar, Navin Khattry, Anant Gokarn, Bhausaheb Bagal, Hasmukh Jain, Sumeet Mirgh, Sridhar Epari, Tanuja Shet, Trupti Pradhan, Shweta Shirsat, Madan Barkume, Caroline Mathen, Poonam Gera, Rohit Kumar Verma, Elveera Saldanha, Pratik Chandrani, Jitendra Gawde, Shubhada Chiplunkar & Jyoti Kode. Defining alteration in bone marrow mesenchymal stem cells (MSC) from acute myeloid leukemia and exploring cultured MSC-conditioned media as a novel anti-leukemia therapy agent. Cancer Immunology Immunotherapy: 2026 Feb 23;75(3):83

2025

• Anthony C, Harish M, Christie J, Venkatraman P (2025). Novel interaction with PSMD9 regulates DNAJA1 turnover and mitochondrial polarity, Biochemical and Biophysical Research Communications.
• Lonare A, Raychaudhuri K, Shah S, Madhu G, Sachdeva A, Basu S, Thorat R, Gupta S, Dalal SN (2025).14-3-3σ restricts YY1 to the cytoplasm, promoting therapy resistance, and tumor progression in colorectal cancer. International Journal of Cancer.
• Jog E, Jainarayanan AK, La Ferlita A, Chakraborty A, Dalwai A, Yahya S, Shivashankar A, Choudhary BS, Chandramouli A, Kazi M, Jain D, Khapare N, B A, Khan BK, Gera P, Patil P, Thorat R, Verma N, Sehgal L, Saklani A, Kamat SS, Dalal SN, Chaudhary N. Inhibiting de novo lipogenesis identifies a therapeutic vulnerability in therapy-resistant colorectal cancer. Redox Biol. 2025 Feb;79:103458. Epub 2024 Dec 11. PMID: 39705849; PMCID: PMC11729006.
• Choudhary BS, Chaudhary N, Khan BK, Vijan A, Mandal D, Pilankar L, Gawand S, Uttankar P, Sharma M, Shivashankar A, Doloi R, Joshi N, Das M, Dalal SN. LCN2 promotes focal adhesion formation and invasion by stimulating c-Src activation. Journal of Cell Science. 138(11): jcs263663. PMID: 40356520
• Jaiswal MA, Karn A, Das A, Kumari A, Tiwari S, Dalal SN. 14-3-3ε inhibits premature centriole disengagement by inhibiting the activity of Plk1 and separase. Journal of Cell Science. 138(14): jcs263808PMID: 40576425
• Chaudhary N, Mandal D, Choudhary B, Patra S, Jain D, Poonia P, Shaikh S, Tekalkar S, Malvankar S, Shivashankar A, Jog E, Pilankar L, Thorat R, Kailje V, Khanna S, Manna S, Khan B, Jadhav A, Sharma K, Ramu S, Sahoo S, Jolly MK, Dalal SN, Sinha S, Nair N, Ruppin E, Lamballe F, Maina F, Verma N. Low-GPX4 drives a sustained drug-tolerant persister state in TNBC by a targetable adaptive FSP1 upregulation. Redox Biology.
• Joshi P, Bane S, Chaturvedi P, Gera P, Waghmare SK (2025).Establishment and characterization of patient-derived tongue squamous cell carcinoma cell lines. Human Cell. 38(4):102.
• Mehta D, Paradkar A, Nayak P, Rekhi B, Mohanty B, Chaudhari P, Waghmare SK. Establishment and molecular characterization of the novel cutaneous squamous cell carcinoma cell line from advanced-stage Indian patient. Human Cell.38(4):108. PMID: 40413685
• Shenoy PS, Dash S, Joshi D, Rekhi B, Gota V, Ray P. Withaferin A Exerts Cytotoxicity in Single/multidrug-resistant gastric and ovarian cancer cells and tumor xenografts through the AKT-NF-κB-STAT3-survivin axis. Molecular Carcinogenesis
• Chaudhary N, Choudhary BS, Shivashankar A, Manna S, Ved K, Shaikh S, Khanna S, Baar J, Dani J, Sahoo S, Soundharya R, Jolly MK, Verma N. EGFR-to-Src family tyrosine kinase switching in proliferating-DTP TNBC cells creates a hyperphosphorylation-dependent vulnerability to EGFR TKI. Cancer Cell International. 25(1):55. PMID: 39972345.
• Ghosh K, Iyer RK, Sood S, Islam MS, Labad JG, Khadilkar RJ. Genetic perturbation of cellular homeostasis regulates integrated stress response signaling to control Drosophila hematopoiesis. Biology Open. 14(7): bio062046. PMID: 40554752
• Tejashree Mahaddalkar, Archisman Banerjee, Madhura Ketkar, Rahul Thorat, Nilesh Gardi & Shilpee Dutt. Oncogene, volume 44, pages2103–2115 April (2025). Aurora Kinase A and B inhibition abrogates ‘Neosis’, a non-mitotic cell division of GBM residual cells and prevents GBM recurrence.
• Chaudhary N, La Ferlita A, Choudhary BS, Jog E, Kazi M, Yahya S, Dalwai A, Ostwal V, Kumar S, Redkar S, Khapare N, Kailaje V, B A, Gera P, Bal M, Verma N, Thorat R, Saklani A, Sehgal L, Dalal SN (2025). Patient-derived Organoids and Xenografts Uncover Therapeutic Vulnerabilities in Colorectal Signet Ring Cell Carcinomas. Clinical Cancer Research

2024

• Choudhary BS, Chaudhary N, Vijan A, Mandal D, Pilankar L, Gawand S, Khan B, Shivashankar A, Doloi R, Joshi N, Das M, Dalal SN (2024). LCN2 promotes focal adhesion formation and invasion by stimulating Src activation. bioRxiv.
• Tilwani S, Gandhi K, Dalal SN (2024). 14-3-3ε conditional knockout mice exhibit defects in the development of the epidermis. FEBS Letters.
• Roy S, Mehta D, Paradkar A, Chovatiya G, Waghmare SK (2024). Dab2 (Disabled-2), an adaptor protein, regulates self-renewal of hair follicle stem cells. Communications Biology. 7(1): 525.
• Roy S, Mehta D, Sawant S, Waghmare SK (2024). Disabled 2 (Dab2) Regulates Tumour Progression in Skin Squamous Cell Carcinoma
• Phadte P, Bishnu A, Dey P, M M, Mehrotra M, Singh P, Chakrabarty S, Majumdar R, Rekhi B, Patra M, De A, Ray P (2024). Autophagy-mediated ID1 turnover dictates chemo-resistant fate in ovarian cancer stem cells. Journal of Experimental and Clinical Cancer Research. 43(1):222

2023

• Christie J, Anthony CM, Harish M, Mudartha D, Farooqee SBUD, Venkatraman P. The interaction network of the proteasome assembly chaperone PSMD9 regulates proteostasis. FEBS Journal.
• Choudhary B, Chaudhary N, Shah M, Dwivedi N, Smitha PK, Das M, Dalal SN. Lipocalin 2 inhibits actin glutathionylation to promote invasion and migration. FEBS Letters.
• Pratiksha Mahadik, Sejal Patwardhan. ECM stiffness-regulated exosomal thrombospondin-1 promotes tunneling nanotubes-based cellular networking in breast cancer cellsArchives of Biochemistry and Biophysics, 742, 109624, (2023) PMID: 37146866
• Joshi P, Waghmare S. Molecular signaling in cancer stem cells of tongue squamous cell carcinoma: Therapeutic implications and challenges. World Journal of Stem Cells 15(5): 438-452.
• Mehta D, Shaikh S, Mohanty B, Chaudhari P, Waghmare SK (2023). Secretory phospholipase (sPLA2-IIA) regulates breast cancer stem cells differentiation and metastatic potential. Biochemical and Biophysical Research Communication. 677:98-104. PMID: 37566923.
• Navarange SS, Bane SM, Mehta D, Shah S, Gupta S, Waghmare SK (2023). Epithelial-to-mesenchymal transition status correlated with ultrastructural features, and TP53 mutation in patient-derived oral cancer cell lines. Molecular Biology Reports. 50(10):8469-8481. PMID: 37639153
• Nazia Chaudhary, Bhagya Shree Choudhary, Anusha Shivashankar, Subhakankha Manna, Khyati Ved, Shagufa Shaikh, Sonal Khanna, Jeetnet Barr, Jagruti Dani, Nandini Verma. EGFR-to-Src family tyrosine kinase switching in proliferating-DTP TNBC cells creates a hyperphosphorylation-dependent vulnerability to EGFR TKI. bioRxiv 2023.07.17.549374;
• Kode, Jyoti; Maharana, Jitendra; Dar, Asif; Mukherjee, Shayanti; Gadewal, Nikhil; Sigalapalli , Dilep; Kumar, Satyanshu; Panda, Debashis; Ghosh , Soumyajit ; Keshry , Supriya ; Mamidi , Prabhudutta ; Chattopadhyay , Soma ; Pradhan, Trupti; Kailaje, Vaishali; Inamdar, Sunil; Gujjarwar , Vidula. 6-Shogaol exhibits anti-viral and anti-inflammatory activity in COVID-19 associated inflammation by regulating NLRP3 inflammasome. ACS Omega 8, 2, 2618–2628
• Low levels of CD26 on certain cellular subtypes of donor harvest is associated with better clinical outcomes post allogeneic stem cell transplantation through regulation of NF-κB pathway and pro-inflammatory cytokines. International Immunopharmacology. 125(Part A): 111054.PMID: 37890379. Kandekar S, Punatar S, Khattry N Gokarn A, Jindal N, Mirgh S, Chichra A, Tembhare P, Rane P, Gawade J, Mathew L, Patil A, Chiplunkar S, Kode J (2023).
• RajeshKumar Meher , Jitendra Maharana , Jeshma Kovvuri , K.Nirmal Kumar , Trupti Pradhan , Vaishali Kailaje , Arvind Ingle , Subrata Sen , Madan Barkume, Meena Patkar, Anand Vaibhaw, ShowkatAhmad Mir, Arpita Ghosh, Sanjeev Kamte, Ahmed Kamal j, Jyoti Kode. A Phenstatin-based indole-chalcone hybrid as novel anti-inflammatory agent targeting the NLRP3 Inflammasome: Leads from computational and preclinical studies
• Dey P, Gadewal N, De A. Pathogenic HER3 dimerization domain mutations create a structural bias towards un-conventional EGFR-HER3 signalling axis in breast cancer. International Journal of Biological Macromolecules.
• Mehta D, Shaikh S, Mohanty B, Chaudhari P, Waghmare SK (2023). Secretory phospholipase (sPLA2-IIA) regulates breast cancer stem cells differentiation and metastatic potential. Biochemical and Biophysical Research Communication. 677:98-104. PMID: 37566923.

2022

• Mal A, Bukhari AB, Singh RK, Kapoor A, Barai A, Deshpande I, Wadasadawala T, Ray P, Sen S, De A. EpCAM-Mediated Cellular Plasticity Promotes Radiation Resistance and Metastasis in Breast Cancer. Frontiers in Cell Developmental Biology. 8:597673 2021 PMID: 33490064
• Bose, A., Modi, K., Dey, S., Dalvi, S., Nadkarni, P. Sudarshan, M., Tapas K. Kundu, T.K., Venkatraman, P., and Dalal, S.N. 14-3-3γ prevents centrosome duplication by inhibiting NPM1 function. Genes to Cells, 26, 426-446 2021).
• Sarika Tilwani, Karan Gandhi, Satya Narayan, Sri Rama Koti Ainavarapu, Sorab Nariman Dalal (2021).Disruption of desmosome function leads to increased centrosome clustering in 14-3-3γ-knockout cells with supernumerary centrosomes.
• Raghavan, R., Koyand, N., Beher, R., Chetlangia, N., Ramadwar, M., Pawade, S., Thorat, R., van Hengel, J., Sklyarova, T., van Roy, F., and Dalal, S.N. (2022). Plakophilin3 loss leads to increased adenoma formation and rectal prolapse in APCmin mice. Biochem. Biophys. Res. Commun.586, 14-19
• N. Chaudhary, N. Joshi, R. Doloi, A. Shivashankar, R. Thorat and S. N. Dalal. (2022). Plakophilin3 loss leads to an increase in autophagy and radio-resistance. Biochem Biophys Res Commun.620, 1-7.
• Singh, Bhawna, et al. The BRCA2 and CDKN1A interacting protein (BCCIP) stabilizes stalled replication forks and prevents degradation of nascent DNA." FEBS letters 596.16 (2022): 2041-2055
• Mulla SW, Venkatraman P. Novel Nexus with NFκB, β-catenin, and RB1 empowers PSMD10/Gankyrin to counteract TNF-α induced apoptosis establishing its oncogenic role. International Journal of Biochemistry & Cell Biology. 146: 106209. PMID: 35378311.
• Mukherjee S, Sakpal A, Mehrotra M, Phadte P, Rekhi B, Ray P (2022). Homo and heterotypic cellular cross-talk in epithelial ovarian cancer impart pro-tumorigenic properties through differential activation of the Notch3 pathway. Cancers. 14(14): 3365.
• Mishra SK, Dhadve AC, Mal A, Reddy BPK, Hole A, CM Krishna, Ray P, Srivastava R, De A. Photothermal therapy (PTT) is an effective treatment measure against solid tumors which fails to respond conventional chemo/radiation therapies in clinic. Biomaterials Advances. 143:213153. PMID: 36343390
• Mukherjee S, Sharma S, Soni V, Joshi A, Gaikwad A, Bellare J, Kode J. Improved osteoblast function on titanium implant surfaces coated with nanocomposite Apatite-Wollastonite-Chitosan- an experimental in-vitro study. Journal of Materials Science: Materials in Medicine. 33(3):25. PMID: 35190908.

2021

• Sheikh Burhan Ud Din Farooqee, Joel Christie, Prasanna Venkatraman. PSMD9 ribosomal protein network maintains nucleolar architecture and WT p53 levels, Biochemical and Biophysical Research Communications, 2021
• Chaudhary N, Choudhary BS, Shah SG, Khapare N, Dwivedi N, Gaikwad A, Joshi N, Raichanna J, Basu S, Gurjar M, Smitha PK, Saklani A, Gera P, Ramadwar M, Patil P, Thorat R, Gota V, Dhar SK, Gupta S, Das M, Dalal SN. Lipocalin 2 expression promotes tumor progression and therapy resistance by inhibiting ferroptosis in colorectal cancer, Int J Cancer.
• Bose A, Modi K, Dey S, Dalvi S, Nadkarni P, Sudarshan M, Kundu TK, Venkatraman P, Dalal SN. 14-3-3γ prevents centrosome duplication by inhibiting NPM1 function. Genes Cells, 26(6):426-446. PMID: 33813791
• Mal A, Bukhari AB, Singh RK, Kapoor A, Barai A, Deshpande I, Wadasadawala T, Ray P, Sen S, De A. EpCAM-Mediated Cellular Plasticity Promotes Radiation Resistance and Metastasis in Breast Cancer. Frontiers in Cell Developmental Biology. 8:597673 2021 PMID: 33490064

2020

• Ramchandra V Amnekar, Shafqat A Khan, Mudasir Rashid, Bharat Khade, Rahul Thorat, Poonam Gera, Shailesh V Shrikhande, Duane T Smoot, Hassan Ashktorab, Sanjay Gupta. Histone deacetylase inhibitor pre-treatment enhances the efficacy of DNA-interacting chemotherapeutic drugs in gastric cancer (2020). World J Gastroenterol 2020 February 14; 26(6): 598-613
• Sanket G. Shah, Tripti Verma, Mudasir Rashid, Nikhil Gadewal, Sanjay Gupta. Histone H2A isoforms: Potential implications in epigenome plasticity and diseases in eukaryotes (2020). J of Biosciences 45:4
• Asmita Sharda, Mudasir Rashid, Sanket Shah, Ajit Kumar Sharma, Saurav Raj Singh, Poonam Gera, Murali Krishna Chilakapati, Sanjay Gupta. Elevated HDAC activity and altered histone phospho-acetylation confer acquired radio-resistant phenotype to breast cancer cells (2020) Clinical Epigenetics. 12:4
• Sanket G. Shah, Tushar Mandloi, Pooja Kunte, Abhiram Natu, Mudasir Rashid, Divya Reddy, Nikhil Gadewal & Sanjay Gupta. HISTome2: a database of histone proteins, modifiers for multiple organisms and epidrugs. Epigenetics & Chromatin; 13: 31 (2020)
• Mukherjee S, Agarwal M, Bakshi A, Sawant S, Thomas L, Fujii N, Nair P, Kode J. Chemokine SDF1 Mediated Bone Regeneration Using Biodegradable Poly(D, L-lactide-co-glycolide) 3D Scaffolds and Bone Marrow-Derived Mesenchymal Stem Cells: Implication for the Development of an “Off-the-Shelf” Pharmacologically Active Construct. Biomacromolecules
• Kode J, Kovvuri J, Nagaraju B, Jadhav S, Barkume M, Sen S, Kasinathan NK, Chaudhari P, Mohanty BS, Gour J, Sigalapalli DK, Kumar CG, Pradhan T, Banerjee M, Kamal A. Synthesis, biological evaluation, and molecular docking analysis of phenstatin based indole linked chalcones as anticancer agents and tubulin polymerization inhibitors. Bioorganic Chemistry.
• Dimri S, Malhotra R, Shet T, Mokal S, Gupta S, De A (2020). Noncanonical pS727 post translational modification dictates major STAT3 activation and downstream functions in breast cancer. Experimental Cell Research. 396(2): 112313. PMID: 33002501
• Rathod M, Kelkar M, Valvi S, Salve G, De A (2020). FOXA1 regulation turns benzamide HDACi treatment effect specific in breast cancer promoting NIS gene mediated targeted radioiodine therapy. Molecular Therapy: Oncolytics. 19: 93-104. PMID: 33102692
• Mal A, Dey P, Hayes RM, McCarthy JV, Ray A, De A (2020). In Silico Identification of Potential Phosphorylation in the Cytoplasmic Domain of Epithelial Cell Adhesion Molecule. ACS Omega. 5(48): 30808-30816. PMID: 33324790
• Ghorai, A., Mahaddalkar, T., Thorat, R. and Dutt, S. (2020). Sustained inhibition of PARP-1 activity delays glioblastoma recurrence by enhancing radiation-induced senescence. Cancer Lett. 490, 44-53.

2019

• Harish M, Kannan S, Puttagunta S, Pradhan MR, Verma CS, Venkatraman P. A Novel Determinant of PSMD9 PDZ Binding Guides the Evolution of the First Generation of Super Binding Peptides. Biochemistry. 58(32): 3422-3433, 2019. PMID: 31287951.
• Sahu I, Nanaware P, Mane M, Mulla SW, Roy S, Venkatraman P. Role of a 19S Proteasome Subunit- PSMD10(Gankyrin) in Neurogenesis of Human Neural Progenitor Cells. Int J Stem Cells. 12(3):463-473, 2019. PMID: 31474027

2018

• Mansa Gurjar, Kumar Krishna Raychaudhuri, Snehal Mahadika, Divya Reddy, Apurva Atak, Trupti Shetty Kruthi Rao, Mansi S. Karkhanis, Prajakta Gosavi, Lalit Sehgal, Sanjay Gupta, Sorab N. Dalal. Plakophilin3 increases desmosome assembly, size and stability by increasing expression of desmocollin2, Biochemical and Biophysical Research Communications, Volume 495, Issue 1, 1 January 2018, Pages 768-774
• Vishal SS, Tilwani S, Dalal SN. Plakoglobin localization to the cell border restores desmosome function in cells lacking 14-3-3γ. Biochem Biophys Res Commun. 495(2): 1998-2003. PMID: 29253567
• Basu S, Chaudhary N, Shah S, Braggs C, Sawant A, Vaz S, Thorat R, Gupta S, Dalal SN. Plakophilin3 loss leads to an increase in lipocalin2 expression, which is required for tumour formation. Exp Cell Res. 369(2): 251-265. PMID: 29803740

2017

• Kelkar MG, Thakur B, Derle A, Chatterjee S, Ray P, De A. Tumor suppressor protein p53 exerts negative transcriptional regulation on human sodium iodide symporter gene expression in breast cancer. Breast Cancer Res Treat. 164(3), 603-615.
• Chovatiya, G.L., Sarate, R.M., Sunkara, R.R., Gawas, N.P., Kala, V., and Waghmare, S.K. Secretory phospholipase A2-IIA overexpressing mice exhibit cyclic alopecia mediated through aberrant hair shaft differentiation and impaired wound healing response. Sci Rep 7, 11830-9, PMID: 28912581
• Rathod M, Chatterjee S, Dutta S, Kalraiya R, Bhattacharya D, De A. Mannose glycosylation is an integral step for human NIS localization and function in breast cancer cells (2019). Journal of Cell Science. PMID: 31455607.

2016

• Mukhopadhyay, A., Sehgal, L., Bose, A., Gulvady, A., Senapati, P., Thorat, R., Basu, S., Bhatt, K., Hosing, A. S., Balyan, R., Borde, L., Kundu, T. K., and Dalal, S. N. 2016. 14-3-3γ prevents centrosome amplification and neoplastic progression. Scientific Reports 6, Article number: 26580 (2016)
• Raychaudhuri, K., Chaudhary, N., Gurjar, M., D'Souza, R., Limzerwala, J., Maddika, S., and Dalal, S. N. (2016). 14-3-3sigma loss leads to activation of the epithelial to mesenchymal transition due to the stabilization of c-Jun. J Biol Chem 291: 16068-16081. (2016).
• Rahul M. Sarate, Gopal L. Chovatiya, Vagisha Ravi, Bharat Khade, Sanjay Gupta, Sanjeev K. Waghmare. sPLA2 -IIA Overexpression in Mice Epidermis Depletes Hair Follicle Stem Cells and Induce Differentiation Mediated through Enhanced JNK/c-Jun Activation. Stem Cells, 34(9):2407-17, 2016

2015

• Chatterjee S, Thaker N, De A. Combined 2-deoxy glucose and metformin improves therapeutic efficacy of sodium-iodide symporter-mediated targeted radioiodine therapy in breast cancer cells. Breast Cancer (Dove Med Press). 7:251-65.
• Basu S, Thorat R, Dalal SN. MMP7 is required to mediate cell invasion and tumor formation upon plakophilin3 loss.
• Shafqat A. Khan, Divya R Velga, Sanjay Gupta. Global Histone Posttranslational Modifications and Cancer: Biomarkers for Diagnosis, Treatment and Prognosis? World Journal of Biological Chemistry, 6(4): 333–345, 2015
• Ajit K. Sharma, Shafqat A. Khan, Asmita Sharda, Divya R Velga, Sanjay Gupta. MKP1 phosphatase mediates G1-specific dephosphorylation of H3Serine10P in response to DNA damage Mutation Research, 778:71-9, 2015
• Ajit K. Sharma, Saikat Bhattacharya, Shafqat A. Khan, Bharat Khade, Sanjay Gupta. Dynamic alteration in H3 serine 10 phosphorylation is G1-phasespecific during ionization radiation induced DNA damage response inhuman cells. Mutation Research, 773: 83–91
• Snehal M.Gaikwad ,BhushanThakur, Asmita Sakpal, Ram K.Singh Pritha Ray. Differential activation of NF-κB signaling is associated with platinum and taxane resistance in MyD88 deficient epithelial ovarian cancer cells

2014

• Sehgal L, Usmani, A. Dalal SN, Majumdar, SS. Generation of transgenic mice by exploiting spermatogonial stem cells in vivo. Methods Mol Biol 1194: 327-337.
• Sehgal L, Mukhopadhyay A, Rajan A, Khapare N, Sawant M, Vishal SS, Bhatt K, Ambatipudi S, Antao N, Alam H, Gurjar M, Basu S, Mathur R, Borde L, Hosing AS, Vaidya MM, Thorat R, Samaniego F, Kolthur-Seetharam U, Dalal SN. 14-3-3gamma mediated transport of plakoglobin to the cell border is required for the initiation of desmosome assembly in vitro and in vivo. J Cell Sci 127: 2174-2188
• Shafqat A Khan, Monica Tyagi, Ajit K Sharma, Savio G Barreto, Bhawna Sirohi, Mukta Ramadwar, Shailesh V Shrikhande, Sanjay Gupta. Cell-type specificity of β-actin expression and its clinicopathological correlation in gastric adenocarcinoma. World Journal of Gastroenterology, 20(34):12202-11. 2014
• Ram K.Singha, Snehal M.Gaikwada, Ankit Jinagera, Smrita Chaudhurya, Amita Maheshwari, Pritha Ray. IGF-1R inhibition potentiates cytotoxic effects of chemotherapeutic agents in early stages of chemoresistant ovarian cancer cells

2013

• Iyer SV, Dange PP, Alam H, Sawant SS, Ingle AD, Borges AM, Shirsat NV, Dalal SN, Vaidya MM. Understanding the role of keratins 8 and 18 in neoplastic potential of breast cancer derived cell lines. PLoS One 8: e53532.
• Chatterjee S, Malhotra R, Varghese F, Bukhari AB, Patil A, Budrukkar A, Parmar V, Gupta S, De A. Quantitative Immunohistochemical Analysis Reveals Association between Sodium Iodide Symporter and Estrogen Receptor Expression in Breast Cancer. PLoS One. 8(1): e54055. [IF 3.53]

2012

• Alam H, Bhate AV, Gangadaran P, Sawant SS, Salot S, Sehgal L, Dange PP, Chaukar DA, D'Cruz A K, Kannan S, Gude R, Kane S, Dalal SN, Vaidya MM. Fascin overexpression promotes neoplastic progression in Oral Squamous Cell Carcinoma. BMC Cancer 12:32.
• Khapare N, Kundu ST, Sehgal L, Sawant M, Priya R, Gosavi P, Gupta N, Alam H, Karkhanis M, Naik N, Vaidya MM, Dalal SN. Plakophilin3 Loss Leads to an Increase in PRL3 Levels Promoting K8 Dephosphorylation, Which Is Required for Transformation and Metastasis. PLoS One 7: e38561.

2011

• Gosavi P, Kundu ST, Khapare N, Sehgal L, Karkhanis MS, Dalal SN. E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly. Cell Mol Life Sci 68:1439-1454.
• Sehgal L, Thorat R, Khapare N, Mukhopadhaya A, Sawant M, Dalal SN. Lentiviral mediated transgenesis by in vivo manipulation of spermatogonial stem cells. PLoS One 6: e21975.
• Telles E, Gurjar M, Ganti K, Gupta D, Dalal SN. Filamin A stimulates cdc25C function and promotes entry into mitosis. Cell Cycle 10:776-782.
• Alam H, Kundu ST, Dalal SN, Vaidya MM. Loss of keratins 8 and 18 leads to alterations in alpha6beta4-integrin-mediated signalling and decreased neoplastic progression in an oral-tumour-derived cell line. J Cell Sci 124:2096-2106.
• Alam H, Sehgal L, Kundu ST, Dalal SN, Vaidya MM. Novel function of keratins 5 and 14 in proliferation and differentiation of stratified epithelial cells. Mol Biol Cell 22:4068-4078.

2009

• Telles E, Hosing AS, Kundu ST, Venkatraman P, Dalal SN. A novel pocket in 14-3-3epsilon is required to mediate specific complex formation with cdc25C and to inhibit cell cycle progression upon activation of checkpoint pathways. Exp Cell Res 315:1448-1457.

2008

• Hosing AS, Kundu ST, Dalal SN. 14-3-3 Gamma is required to enforce both the incomplete S phase and G2 DNA damage checkpoints. Cell Cycle 7:3171-3179.
• Kundu ST, Gosavi P, Khapare N, Patel R, Hosing AS, Maru GB, Ingle A, Decaprio JA, Dalal SN. Plakophilin3 down regulation leads to a decrease in cell adhesion and promotes metastasis. Int J Cancer 123:2303-2314.
• Garg R, Gupta S and Maru G B. Dietary curcumin modulates transcriptional regulators of phase I and phase II enzymes in benzo[a]pyrene-treated mice: mechanism of its anti-initiating action. Carcinogenesis. 29(5):1022-1032

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If your research has benefited from the support and assistance provided by the Digital Imaging Facility, we kindly request you to acknowledge the Facility in your publications, presentations, theses, and posters.

Facility Email:adif@actrec.gov.in
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MULTIMODE MICROPLATE READER - BIOTEK CYTATION 5

A multimode microplate reader is a versatile laboratory instrument that analyzes multiple samples simultaneously in a microplate using multiple detection methods. These complex optical instruments are essential for high throughput screening in the life sciences and pharmaceutical research enabling researchers to quantify biological and chemical reactions and properties in various assays, including drug discovery, cell proliferation, and gene expression

Technical Features:

MULTIMODE MICROPLATE READER - BMG LABTECH CLARIOSTAR PLUS

A wide range of life science applications, including cell based assays, metabolic analysis (oxygen consumption, pH, ROS), enzymatic assays, quantification (RNA, protein), and reporter gene assays. It offers flexibility through high performance monochromator and tunable dichroic mirrors, a dedicated spectrometer for high resolution spectral scanning and atmospheric control to simulate physiological conditions.

Technical Features:

MICROPLATE READER - BIOTEK EPOCH 2

A microplate reader is a laboratory instrument that quantifies chemical, biological, or physical changes within the wells of a microplate, a multi-well plate used for high-throughput screening. It detects and measures optical signals like absorbance to analyze multiple samples simultaneously, saving time and costs for researchers in the life sciences and pharmaceutical industries.

Technical Features:

MICROLITER SPECTROMETER - DENOVIX DS11+

A microliter spectrophotometer is used to quickly measure the concentration and purity of biological samples, such as DNA, RNA, and proteins, by analysing their light absorbance in just 1-2 microliters of liquid. It works by placing a small sample onto a measurement pedestal, where surface tension forms a liquid column between two fibre optics that passes light through it. The instrument then measures the light absorbed at various wavelengths, which indicates the sample's concentration and the presence of contaminants, allowing researchers to assess sample quality for experiments.

CHEMIDOC IMAGING SYSTEMS - BIO RAD MP

The ChemiDoc imaging system from Bio Rad is used to image and document gels and western blots. The ChemiDoc imaging system is compatible with a wide range of gel stains, including Ethidium bromide, SYPRO Ruby, Coomassie, and Silver stains. The ChemiDoc Imaging System provides fast, reliable, and sensitive imaging and documentation of gels and Chemiluminescence western blots. This system is compatible with stain free technology.

Technical Features:

GELDOC IMAGING SYSTEMS - BIO RAD GO IMAGING

The GelDoc Go Imaging System gives you a benchtop imaging solution in a compact and evolved package Acquire high resolution, publication quality images of both nucleic acid and protein gels

Technical Features:

SHAKER INCUBATOR - LAB COMPANION ISS 3075R

Best space efficient model with double stacking Up to 80°C, 500 rpm enables setting of various experimental conditions. Utilized as an incubator using the shelves Three operating modes Temperature Shaking, Temperature, or Shaking Includes internal power outlet Shaking structure with minimized vibration.

Technical Features:

SHAKER INCUBATOR - INNOVA 44R

A Lab Companion incubator shaker is a laboratory instrument that controls temperature and provides mechanical agitation for incubating samples like microorganisms, cells, or enzymes. These devices are used in fields such as biotechnology and microbiology to facilitate growth and cultivation by providing a controlled environment for heating and shaking at user set speeds and temperatures.

Technical Features:

PCR MACHINE (THERMAL CYCLER) - HIMEDIA AND PROFLEX

A PCR machine amplifies specific DNA or RNA segments from samples, which is essential for applications like detecting genetic diseases, identifying pathogens (e.g. viruses), forensic DNA analysis, paternity testing, and gene cloning. It achieves this by rapidly cycling through controlled temperature changes to replicate target genetic material, a process known as Polymerase Chain Reaction (PCR)

Technical Features:

REAL TIME PCR SYSTEM - QUANT STUDIO 5

The Applied Bio systems Quant Studio 5 Real Time PCR System is designed for Proven OptiPlex technology (six decoupled channels/ 21 filter combinations with white LED) yield optimum results.

Technical Features:

PROBE SONICATOR - BRANSON 450 DIGITAL

A probe Sonicator is a laboratory instrument that uses high frequency ultrasonic energy to process liquid samples, commonly for disruption, dispersion, and mixing. It works by converting electrical energy into intense vibrations transmitted through a metal probe directly into the liquid, creating cavitation bubbles that cause intense shearing and mixing. This allows for applications like breaking open cells, preparing nanomaterials, and creating emulsions.

Technical Features:

DIAGENODE SONICATION SYSTEM - BIORUPTOR PICO

A Bioruptor is a laboratory device that uses focused ultrasound and controlled cavitation to create mechanical stress, primarily for shearing biomolecules like DNA and chromatin, but also for disrupting cells, homogenizing tissue, dispersing chemical components, and reducing the size of liposomes.

Technical Features: